Elsevier

Cellular Immunology

Volume 88, Issue 1, 1 October 1984, Pages 213-221
Cellular Immunology

Short communication
Unsaturated fatty acids stimulate NADPH-dependent superoxide production by cell-free system derived from macrophages☆

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Abstract

Arachidonic acid (C20:4) and other unsaturated fatty acids are shown to activate superoxide (O2−) production in a cell-free system represented by sonically disrupted guinea pig peritoneal macrophages. The reaction requires a heat-sensitive cellular component and NADPH, is enhanced by flavin adenine dinucleotide (FAD), and is not linked to enzymatic oxidation of the fatty acid. C20:4-elicited O2− formation is dependent on the cooperation between a subcellular component sedimentable at 48,000g (probably containing the O2−-forming enzyme) and a cytosolic factor. This appears to be the first report of O2− generation being elicited in a cell-free system derived from unstimulated cells and supports the idea that unesterified unsaturated fatty acids act as second messengers of O2− formation in intact phagocytes.

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    ☆

    Supported by Grant 2730 from the U.S.-Israel Binational Science Foundation.

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