Metal binding properties of chondroitin sulphate

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Abstract

The interations of H+, Ca2+, Y3+, La3+, with chondroitin sulphate of cartilage have been studied using a micropotentiometric titration technique. These ions are bound to the carboxyl groups of chondroitin sulphate by the formation of 1:1 complexes, with intrinsic association constants in KCl solution (with the ionic strength in parentheses); H+ log K = 3.06 ± 0.02 (0.1); 2.92 ± 0.02 (1.0); Ca2+ log K = 0.42 ± 0.09 (1.0); Y3+ log K = 0.61 ± 0.05 (1.0); La3+ log K = 0.79 ± 0.03 (1.0). The electrostatic contribution to the binding constant, due to the charge of the polymeric molecule, accords with the “nearest-neighbour interaction” description of the titration curve. The influence of the sulphate groups on these results is discussed and is considered small.

References (21)

  • I. Malawista et al.

    J. Biol. Chem.

    (1958)
  • K. Meyer et al.

    Biochim. Biophys. Acta

    (1956)
  • J. Einbinder et al.

    J. Biol. Chem.

    (1950)
  • M.B. Mathews

    Biochim. Biophys. Acta

    (1959)
  • I.G.F. Gilbert

    Biochim. Biophys. Acta

    (1960)
  • E.A. Davidson et al.

    J. Am. Chem. Soc.

    (1955)
  • A.G. Lloyd et al.

    Nature

    (1959)
  • J.T. Irving

    Calcium Metabolism

  • J.M. Vaughan
  • M. Stacey et al.

    J. Chem. Soc.

    (1955)
There are more references available in the full text version of this article.

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