Plantagoside, a novel α-mannosidase inhibitor isolated from the seeds of Plantago asiatica, suppresses immune response

doi:10.1016/0006-291X(89)92743-5

Biochemical and Biophysical Research Communications

Volume 165, Issue 3, 30 December 1989, Pages 1292-1298

https://doi.org/10.1016/0006-291X(89)92743-5 Get rights and content

Abstract

A hot-water extract from the seed of Plantago asiatica showed a potent inhibitory activity against jack bean α-mannosidase, and a flavanone glucoside, plantagoside, was isolated as the inhibitor. Plantagoside was a specific inhibitor for jack bean α-mannosidase (IC₅₀ at 5 μM) and appeared to be a non-competitive inhibitor of the enzyme. Whereas, negligible or weak inhibitory activities were observed for β-mannosidase, β-glucosidase, and sialidase tested. Plantagoside also inhibited α-mannosidase activities in mouse liver lysosomal and microsomal fractions, and the enzyme inhibitory activity in microsoma fraction was enhanced in the presence of glucosidase inhibitor, castanospermine. Plantagoside suppressed antibody response to sheep red blood cells and concanavalin A induced lymphocyte proliferation which was measured by [³H]thymidine incorporation.

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Plantagoside, a novel α-mannosidase inhibitor isolated from the seeds of Plantago asiatica, suppresses immune response

Abstract

Trends Biochem. Sci

Methods Enzymol

Arch. Biochem. Biophys

Biochem. Biophys. Res. Commun

Annu. Rev. Biochem

Annu. Rev. Biochem

Chem. Pharm. Bull