Inactivation of hepatic cytochrome P-450 by allenic substrates

doi:10.1016/0006-291X(80)91251-6

Biochemical and Biophysical Research Communications

Volume 94, Issue 2, 30 May 1980, Pages 443-449

https://doi.org/10.1016/0006-291X(80)91251-6 Get rights and content

Abstract

Hepatic microsomal cytochrome P-450 from phenobarbital-pretreated rats is destroyed by 17-α-propadienyl-19-nortestosterone, 1-propadienylcyclohexanol, and 1,1-dimethylallene. Substantial activity is also exhibited by 1-propadienylcyclohexanol against the enzymes in microsomes from 3-methylcholanthrene pretreated rats. The destructive process requires NADPH, is not inhibited by glutathione, and is paralleled by equimolar loss of hepatic heme but not by $in$ $vivo$ accumulation of identifiable “green” pigments. Attempted metabolism of allenes can thus result in destruction of cytochrome P-450.

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Inactivation of hepatic cytochrome P-450 by allenic substrates

Abstract

Chem. Biol. Interact

Biochem. Biophys. Res. Commun

Biochem. Pharmacol

Biochem. Pharmacol

Biochem. Pharmacol

J. Biol. Chem

Biochem. Biophys. Res. Commun

Biochem. Pharmacol

Biochem. J

Science

Res. Commun. Chem. Pathol. Pharmacol

Molec. Pharmacol