A radiometric assay for hepatic epoxide hydrase activity with [7-3H] styrene oxide☆
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2005, Methods in EnzymologyCitation Excerpt :The drawback, however, is that the radiolabeled compound required for this procedure is not usually available from commercial sources and has to be prepared in advance or ordered as a custom synthesis. The preparation itself is not complicated because the compound can be obtained in a single step from radiolabeled styrene by turnover with m‐chloroperoxybenzoic acid in methylene chloride and subsequent purification of the product (Oesch et al., 1971). The use of chloroform as the organic solvent has two advantages that made it difficult to substitute it against a less harmful chemical in the present protocol.
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A preliminary report of this work has appeared: Federation Proc., 29 (1970) 473.
Copyright © 1971 Published by Elsevier B.V.