Voltage-induced thickness changes of lipid bilayer membranes and the effect of an electric field on gramicidin A channel formation
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Cited by (49)
Effect of electric field on structure and dynamics of bilayers formed from anionic phospholipids
2014, Electrochimica ActaCitation Excerpt :Studies of the electrical properties of lipid membranes have traditionally been carried out using patch clamp capacitance measurements [3,4], conductivity, capacitance and ac impedance of bilayer phospholipid membranes [5–10]. The action of ion channel-forming peptides can also be studied in this way [5,9,10]. A different approach to the study of phospholipid films has involved supporting a lipid monolayer on a mercury drop electrode, which is pushed through a phospholipid monolayer on an aqueous sub-phase [11–20].
Role of the transmembrane potential in the membrane proton leak
2010, Biophysical JournalCitation Excerpt :The molecular consequence of applying an electric field to the lipid membrane is poorly understood. It has been shown that an electric field creates a compressive stress in the membrane (63,64), however, the amplitude of such compression is rather small (65,66). ATR-FTIR, ESR, and 31P NMR studies with multilayers showed that the field influences the orientation of polar headgroups but without significant effects on the structure and dynamics of the hydrocarbon chains (67,68).
Sensitized Photoinactivation of Gramicidin Channels: Technique and Applications
2005, Advances in Planar Lipid Bilayers and LiposomesCitation Excerpt :Actually a change in the voltage can modulate not only the rate constants of gramicidin channel formation and dissociation but also some properties of the lipid bilayer, thereby altering the kinetic pattern [74]. Bamberg and Lauger [75] noted that the current relaxation kinetics became nonexponential, if a membrane torus constituted more than 10% of the total membrane area. As shown in Ref. [76], the time course of the gramicidin-mediated current relaxation after a voltage jump contained a slow phase with a characteristic time of 7 s additionally to a usual phase with that of 1 s.
Impedance Analysis and Single-Channel Recordings on Nano-Black Lipid Membranes Based on Porous Alumina
2004, Biophysical JournalCitation Excerpt :Bathed in 0.5 M KCl electrolyte solution, the different opening states of individual channels (O1–5O1) were clearly discernable, corroborating the idea that single lipid bilayers have been formed on the porous support as it is well-established that gramicidin ion channels can only be observed in single lipid bilayers. The mean conductance of ∼60 pS applying a holding potential of 70 mV is in the conductance range reported by others (Andersen, 1983; Bamberg and Benz, 1976; Bamberg et al., 1976; Hladky and Haydon, 1972; Myers and Haydon, 1972). No channel activity was observed before adding the peptide to the solution.
Gating Gramicidin Channels in Lipid Bilayers: Reaction Coordinates and the Mechanism of Dissociation
2004, Biophysical JournalCitation Excerpt :Less attention has been paid to channel gating: the energetics and the “reaction coordinate” for dissociation and formation of the gA dimer. A number of studies have demonstrated the dependence of the formation and dissociation rates of gA channels on voltage (Bamberg and Benz, 1976; Sandblom et al., 2001), membrane thickness (Kolb and Bamberg, 1977; Elliot et al., 1983), ion concentration (Ring and Sandblom, 1988; Ring, 1992), and elastic properties of lipid bilayers (Neher and Eibl, 1977; Ring, 1996; Goulian et al., 1998; Lundbæk and Andersen, 1999). To understand the origin of these various phenomena, a molecular mechanism for dimer formation and dissociation in lipid bilayer membranes is needed.
Membrane molecule reorientation in an electric field recorded by attenuated total reflection Fourier-transform infrared spectroscopy
2001, Biophysical JournalCitation Excerpt :The electric field creates a compressive stress in the membrane that can lead to changes in the dimensions of membrane structure (Coster, 1975). The voltage-induced thickness change (compression) of planar black lipid membranes on DOPC has been carefully investigated by Bamberg and Benz (1976) through capacity changes. However, the presence of n-decane in these experiments may have affected the results.