Enhanced migration of glucose from water into chloroform in presence of phospholipids

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Abstract

The necessity for the exclusion of water in the preparation of previously reported sugar-phospholipid complexes in highly nonpolar vehicles was found not to apply when chloroform was used as the organic solvent. The apparent chloroform/water partition coefficient of glucose was markedly raised by addition to the chloroform of any of the major fractions of the phospholipids extracted from human erythrocyte stromata, but not by the neutral lipids. This induced migration of the sugar was not accompanied by comparable entrapment of water in the organic phase; sugar levels in the chloroform could attain more than 200 times the control figure, resulting even in higher glucose/water molecular ratios than in the aqueous phase. Characteristic quantitative differences among the major classes of phospholipids in respect to this sugar interaction were apparent. The extent of the sugar solubilization from a given aqueous solution by a given phospholipid preparation was directly proportional to the concentration of lipid throughout the range tested (up to nearly 10 mm); however, no evidence of approaching saturation was seen even at glucose levels exceeding 1.5 M.

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    This work was supported by grants from the National Science Foundation (GB-2979), the National Institute of Arthritis and Metabolic Diseases (5-RO1-AM-07175), and by a U.S.P.H.S. Research Career Development Award (No. 5-K3-AM-15,469). Human blood was obtained through Grant No. G-1–60/S3 from the American National Red Cross Blood Program.

    2

    Dr. Jung's present address is State University of New York at Buffalo, Department of Biophysics, and Veterans Administration Hospital, 3495 Bailey Avenue, Buffalo, New York 14215.

    3

    Dr. LeFevre's present address is State University of New York at Stony Brook, Health Sciences Center, Stony Brook, New York 11790.

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