Stimulation of carotene synthesis in submerged cultures of Neurospora crassa by surface-active agents and ammonium nitrate

https://doi.org/10.1016/0003-9861(60)90197-1Get rights and content

Abstract

A submerged “shake-type” procedure was developed for the culture of Neurospora crassa (cross of wild types E 5297a and E 5256A) and has given higher carotene production than methods which have been previously described. The submerged cultures, grown in the presence of Tween 40 on a platform shaker, gave maximal pigmentation of conidia-free mycelia in approximately 512 days. Stimulation of carotene synthesis was maximal at 0.8% with Tween 40. Some other surface agents were tried, and only the Tweens gave good submersion and dispersion of the mycelium. In a 2% sucrose medium, addition of ammonium nitrate produced the highest carotene yield at a carbon to nitrogen ratio of 4.

References (5)

  • M. Zalokar

    Arch. Biochem. Biophys

    (1954)
  • F. Haxo

    Arch. Biochem

    (1949)
There are more references available in the full text version of this article.

Cited by (0)

Journal Paper No. 1487 of the Purdue Agricultural Experiment Station, Lafayette, Indiana.

2

Present address: American Meat Institute Foundation, 939 E. 57th Street, Chicago 37, Ill.

View full text