Renaturation of dextranase activity from culture supernatant fluids of Streptococcus sobrinus after sodium dodecylsulfate polyacrylamide gel electrophoresis
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2018, New and Future Developments in Microbial Biotechnology and Bioengineering: Actinobacteria: Diversity and Biotechnological ApplicationsBiocatalytic potential of an alkalophilic and thermophilic dextranase as a remedial measure for dextran removal during sugar manufacture
2012, Bioresource TechnologyCitation Excerpt :The gel was silver stained by the method of Merril et al. (1983). Dextranase activity was also detected on an 8.0% SDS–PAGE with blue-dextran (0.5%) as previously described by Barrett and Curtiss (1986). Isoelectric focusing (IEF) was carried out on a 17.0 cm, pH 3.0–10.0 Readystrip™ IPG strip and run in a Protean-IEF cell (Biorad laboratories Inc., USA) along with standard pI marker (IEF mix 3.6–9.3: I3018, Sigma–Aldrich, USA) according to manufacturer’s instructions.
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1997, Enzyme and Microbial TechnologyCloning and expression in Escherichia coli of dextranase genes from Bacteroides thetaiotaomicron
1991, FEMS Microbiology Letters
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