Affinity partitioning with polymer-bound Cibacron blue F3G-A for rapid, large-scale purification of phosphofructokinase from baker's yeast
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2000, Journal of Biological ChemistryCitation Excerpt :Segregants were obtained after tetrad analysis (Table I, available as supplemental material in on-line version) and used for further physiological characterizations. Pfk was purified for determination of kinetic parameters using a modification of the method of (32), based on precipitation with polyethylene glycol and affinity purification on Affi-Gel Blue columns, as described (11). Activation by fructose 2,6-bisphosphate was determined in 50 mm sodium phosphate buffer at pH 7.0, containing 10 mm magnesium chloride, with 3 mm ATP and 0.5 mm fructose 6-phosphate in a standard assay mixture containing 0.2 mm NADH, 0.3 units of aldolase, 1.5 units of triosephosphate isomerase, and 0.5 units of glycerol-3-phosphate dehydrogenase without other effectors.
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2000, Separation Science and TechnologyEffects of specific binding reactions on the partitioning behavior of biomaterials
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