Solubilization of porcine intestinal α-glucosidases and evidence for the separate identities of isomaltase and limit dextrinase

doi:10.1016/0003-2697(77)90181-6

Analytical Biochemistry

Volume 82, Issue 2, October 1977, Pages 435-444

https://doi.org/10.1016/0003-2697(77)90181-6 Get rights and content

Abstract

The results of a detailed comparison of the relative efficiencies of different procedures for solubilizing the membrane-bound α-glucosidases in hog intestinal mucosa are reported. Procedures for the selective solubilization of certain members of the complex group of α-glucosidases have been developed. The selective solubilization achieved permits the conclusion that separate α-glucosidases are involved in the hydrolysis of isomaltose and the α-limit dextrins formed from starch by α-amylase.

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^☆: Part II in the series Gastrointestinal Degradation of Starch. For Part I, see Ref. 1.

²: Investigator of Howard Hughes Medical Institute.

³: Present address: Regional Hormone Laboratory, 12 Bristo Place, Edinburgh EH1 1ED, Scotland.

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Solubilization of porcine intestinal α-glucosidases and evidence for the separate identities of isomaltase and limit dextrinase☆

Abstract

Anal. Biochem

J. Biol. Chem

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biochim. Biophys. Acta

J. Biol. Chem

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Methods Enzymol