Elsevier

Analytical Biochemistry

Volume 18, Issue 2, February 1967, Pages 248-255
Analytical Biochemistry

Evidence for possible nonspecific reactions between N-ethylmaleimide and proteins

https://doi.org/10.1016/0003-2697(67)90007-3Get rights and content

Abstract

The reaction between NEM and the two proteins lysozyme and ribonuclease A results, under certain experimental conditions, in the alkylation of ϵ-amino and imidazole groups. The extent of reaction may be determined by amino acid analysis since the reaction products yield ninhydrin-positive zones.

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This work was supported by Cancer Research Funds of the University of California.

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