Elsevier

Analytical Biochemistry

Volume 56, Issue 2, December 1973, Pages 361-369
Analytical Biochemistry

A high resolution PAS stain for polyacrylamide gel electrophoresis

https://doi.org/10.1016/0003-2697(73)90202-9Get rights and content

Abstract

An improved PAS method for the detection of glycoproteins after electrophoresis on acrylamide or urea-acrylamide gels is described. Stronger oxidizing conditions and a more controlled washing for the removal of periodic acid resulted in both increased staining intensity and band resolution. The method will not stain protein and it appears that a 2–3 μg sample of bound or precipitable carbohydrate would easily be detected. Mucopolysaccharides were not detectable by this method.

Reference (14)

  • KeyserJ.W.

    Anal. Biochem.

    (1964)
  • CaldwellR.C. et al.

    Arch. Biochem. Biophys.

    (1965)
  • BerensonG.S. et al.

    J. Ather. Res.

    (1966)
  • ZachariusR.M. et al.

    Anal. Biochem.

    (1969)
  • DawesC.

    Arch. Oral. Biol.

    (1965)
  • LowryO.H. et al.

    J. Biol. Chem.

    (1951)
  • RayT.K. et al.

    Biochim. Biophys. Acta

    (1971)
There are more references available in the full text version of this article.

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