A new, sensitive determination of phosphate
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2017, Annals of Agricultural SciencesCitation Excerpt :After that, distilled water was added to raise the volume of this extract to 100 ml; and the contents of P, K, Na, Mn, Zn and Fe were determined. To determine nutrients, the Kjeldahl method (for N), the method of Eible and Lands (for P), the flame photometry method (for K and Na) and a complex ometric titration method (for Ca and Mg) were applied; and an atomic absorption spectrophotometer (Model: AA-670, Shimadzu Co., Kyoto, Japan) was employed to measure the Mn, Zn, and Fe contents (Eibl and Lands, 1969). The total phenolic content was determined using the Folin–Ciocalteu method (Singh et al., 2002).
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2017, ToxiconCitation Excerpt :LAAOs activity was determined according to the method previously reported in the literature (Campos et al., 2013). And 5'-nucleotidase activity was measured by the method previously described by Eibl and Lands (1969) and Katrin Trummal (Trummal et al., 2015) using 5'-AMP as substrate. The catalytic hydrolysis of azocasein by NnNV metalloproteinases were measured in a kinetic way by assuming that the catalytic reaction fits the single enzyme model of Michelis–Menten kinetics.