Fluorescent products of the reaction for the determination of catecholamines with 1,2-diphenylethylenediamine
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Simultaneous determination of 5-hydroxyindoles and catechols from urine using polymer monolith microextraction coupled to high-performance liquid chromatography with fluorescence detection
2009, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life SciencesSimultaneous determination of serum concentrations of levodopa, dopamine, 3-O-methyldopa and α-methyldopa by HPLC
2008, Biomedicine and PharmacotherapyCitation Excerpt :Numerous techniques including radioenzyme [11,12] and chromatographic [13] methods are available for the analysis of these compounds in biological fluids. Their plasma concentrations have been measured by high performance liquid chromatography (HPLC) using UV [14], mass [15,16], electrochemical [17–20] and fluorescence [21–29] detection. Two fluorimetric methods are largely used for the determination of catechols [30]: fluorescence detection after derivatisation with a fluorescent reagent, 1,2-diphenylethylenediamine (DPE) [10,21–26], and native fluorescence detection [27–29].
Chapter 2 Selective Fluorogenic Derivatization of 3-Nitrotyrosine and 3,4-Dihydroxyphenylalanine in Peptides: A Method Designed for Quantitative Proteomic Analysis
2008, Methods in EnzymologyCitation Excerpt :Our objective was the design of a method that would (i) show selectivity toward 3-NY and DOPA-containing peptides and proteins, (ii) yield a fluorescent reaction product with excitation and emission properties far from those of native proteins and/or common oxidation products (e.g., bityrosine, N-formylkynurenin), (iii) be suitable for isotopic labeling for relative quantitation, and (iv) be suitable for the introduction of additional ligands for the improvement of chromatographic behavior and/or affinity enrichment. Based on previous reports that the reaction of catechols with diphenylethylenediamine yielded a fluorescent benzoxazole-type product (Nohta et al., 1992), we focused our efforts toward the reaction of catechols and aminophenols with benzylamine and benzylamine derivatives. These reactions utilize both aromatic substituents (the two hydroxyl groups in the case of DOPA, and the hydroxyl and amino group in the case of 3-aminotyrosine) for the generation of a fluorescent benzoxazole, while no fluorescent benzoxazole product can be generated if one of these substituents would be absent, that is, in the case of Tyr.
Chemiluminescence determination of catecholamines in human blood plasma using 1,2-bis(3-chlorophenyl)ethylenediamine as pre-column derivatizing reagent for liquid chromatography
2000, Analytica Chimica ActaCitation Excerpt :In the PO-CL system, bis[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl]oxalate (TDPO) was used to generate excited dioxetane-1,2-dione which could stimulate the luminophore (Fig. 1). The luminophore in Fig. 1 should be produced as described in the previous paper [28]. The high sensitivity and selectivity of this method allowed the determination of CAs in 20 μl of human blood plasma.