Immunoperoxidase localization of papillomavirus antigens in cervical dysplasia and vulvar condylomas

doi:10.1016/0002-9378(81)90087-9

American Journal of Obstetrics and Gynecology

Volume 140, Issue 8, 15 August 1981, Pages 931-935

https://doi.org/10.1016/0002-9378(81)90087-9 Get rights and content

Abstract

Biopsies of 50 cases of cervical dysplasia (46 mild and 4 moderate) and 40 cases of vulvar condyloma acuminata (genital warts) were screened for the presence of papillomavirus antigens by means of a peroxidase-antiperoxidase method having immunospecificity against the genus-specific (common) antigen(s) of the papillomaviruses. With the use of this technique, on formalin-fixed, paraffin-embedded tissue, papillomavirus antigens were detected in cells with cytologic and histologic features of wart virus infection (so-called koilocytotic atypia). Cells showing a positive reaction for papillomavirus antigens were identified in 24 of 50 (48%) cases of cervical dysplasia and in 20 of 40 (50%) cases of vulvar condyloma. The results of this study provide specific confimation of the presence of papillomavirus antigens in cervical dysplasia and suggest that the papillomavirus may be an important factor in the etiology of this disease.

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^☆: Supported by the Clark Dunlap Cancer Research Fund, University Hospitals of Cleveland.

^∗: Present address: Loyola-Stritch School of Medicine, Maywood, Illinois 60153.

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Immunoperoxidase localization of papillomavirus antigens in cervical dysplasia and vulvar condylomas☆

Abstract

A comparison review of key epidemiological studies in cervical cancer related to current searches for transmissible agents

Cancer Res

Human cervical cancer as a venereal disease

Cancer Res

Immunofluorescent detection of herpesvirus antigens in exfoliated cells from human cervical carcinoma

Condylomatous lesions of the cervix. II. Cytologic, colposcopic, and histopathologic study

Acta Cytol

Electron microscopic detection of papillomavirus particles in selected koilocytotic cells in a routine cervical smear

Acta Cytol

Noncondylomatous cervical wart virus infection

Obstet. Gynecol

Colpocytohistological aspects of uterine condyloma

Gynecologie

Anogenital warts contain several distinct species of human papillomavirus

J. Virol

Viral sequences related to a human skin papillomavirus in genital warts

Nature (Lond.)