InfertilityDoes Bone Marrow–derived Mesenchymal Stem Cell Transfusion Prevent Antisperm Antibody Production After Traumatic Testis Rupture?
Section snippets
Subjects and Methods
The study was approved by local ethics committee (Trauma Research Center, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran). The chemicals consumed in this study were purchased from Sigma-Aldrich (Pool, UK) unless stated otherwise. Animal surgical procedure and maintenance was carried out under supervision of Veterinary Medicine Faculty of University of Tehran guidelines, and ethical approval was obtained from the ethics scrutiny committee before conduction of the experiments.
Results
Six cases were lost during the study: 4 because of anesthesia recovery failure (3 from group 1 and 1 from group 2) and 2 other died during 3 first postoperative days, 1 mouse each from group 1 and 2.
All 10 negative control mice (group 0a,b) were ASA negative and negative for GFP-stained cells regarding lung and testis tissues. ASA production rate was 87.5% (14 of 16) in cell therapy control mice (group1) but only 6 of 18 mice (33.3%) that received MSCs (group 2) had ASA in their serum (P
Comment
During the past few years, a body of evidence is growing on immunomodulatory effects of MSCs,8, 17, 18, 19 which were first discovered as a suppressing effect on T-cell proliferation by means of soluble factors that refrains the pathway somewhere before the IL2 action, which are best reviewed in Abdi et al work (2008) that are believed to include transforming growth factor beta family and IL10. Besides, it has been shown that MSCs lack of costimulatory surface antigen in interaction with T-cell
Conclusion
This study yields that, even in an allogeneic setting, bone marrow–derived MSC transfusion exerted immunosuppressive effects on antibody production in an in vivo model. These results may facilitate the current attempts to reveal the immunomodulatory pathways of MSCs and help establishing a novel clinical application for MSCs in future.
Acknowledgments
Authors wish to thank Dr. Mohammad Ghodsi (Sina Trauma and Surgery Research Center) and Mazdak Salavati for reviewing the manuscript, and staff of Sina Hospital Pathology Laboratory for their kind cooperation, especially Dr. Moradi and Mrs. Nikie.
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Financial Disclosure: The authors declare that they have no relevant financial interests.
Funding Support: This work was funded by the kind contribution of Dr. Naser Ahmadbeigi (Sabz Biotech Ltd) and Tehran University Trauma Research Center.