Original ContributionProtective effects of hemoglobin-based oxygen carrier given to isolated heart during ischemia via attenuation of mitochondrial oxidative damage
Section snippets
Materials and methods
All experiments were approved by the Institutional Animal Care and Use Committee of Sichuan University and conformed to the Guide for the Care and Use of Laboratory Animals published by the U.S. National Institutes of Health (NIH Publication No. 85-23, revised 1996).
Oxygen releasing capacity of HBOC
As shown in Fig. 2A, the O2 releasing capacity of 0. 1 g Hb/dl HBOC was nearly 1.5-fold higher than that of the STS alone (206.70 ± 14.20 vs 139.27 ± 10.83 mm Hg/g wet wt, P < 0.05). Consistently, the cardiac lactic acid release of the WI + HBOC and HS + HBOC groups hearts was largely inhibited compared with the WI and HS groups (P < 0.05 and P < 0.05, respectively, Fig. 2B).
HBOC improves cardiac LV function recovery after I/R
Measures reflecting LV contractile function were similar under basal conditions. During the reperfusion, the WI + HBOC group hearts
Discussion
This study provides distinct evidence that HBOC significantly improved cardiac function and decreased myocardial infarct size, thus exhibiting profound protection against myocardial I/R injury. Such protection was further proven by the marked decreases in myocardial necrosis and apoptosis. To reveal the underlying mechanism, we investigated the redox status of the mitochondria in these hearts. The results clearly showed that the mitochondrial Eh was well preserved by HBOC, which was accompanied
Acknowledgments
This study was supported by grants from the National Nature Science Foundation of China (30772084 and 30828030 to Dr. Jin Liu and 30801083 to Dr. Ronghua Zhou), the Key Project of the Chinese Ministry of Education (106132 to Dr. Ke Dian), and the 973 Program (2005 CB522601 to Dr. Jin Liu). The authors thank Professor Chengmin Yang for technical assistance.
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These authors contributed equally to this study.