Characterisation of human dental stem cells and buccal mucosa fibroblasts
Section snippets
Materials and methods
Cell preparation and culture. Human impacted third molars were obtained during odontectomy with informed consent at the Finnish Student Health Services. A buccal mucosa sample was obtained from one patient during maxillofacial surgery at the Tampere University Hospital, Tampere, Finland. The study was conducted in accordance with the Ethics Committee of the Pirkanmaa Hospital District, Tampere, Finland. DPSCs were isolated from impacted third molars of 10 patients, DFCs from 9 patients, and
Results and discussion
The dental tissue cell sources and BMFs were analysed with FACS to compare surface marker expression characteristics (Table 2). The FACS analysis demonstrated that all three dental cell sources and BMFs showed positive expression for the putative stem cell marker STRO-1; adhesion molecules CD9, CD29, CD49d, CD105, CD106, and CD166; receptor molecule CD44; surface enzymes CD10 and CD13, extracellular matrix protein CD90; complement regulatory protein CD59; and hFSP. All cell sources lacked
Acknowledgments
The authors thank Ms. Miia Juntunen for technical assistance, Mr. Henrik Mannerström for graphic processing consultation, and Mrs. Heini Huhtala for statistical counselling. The work was supported by TEKES, the Finnish Funding Agency for Technology and Innovation, the competitive research funding of the Pirkanmaa Hospital District, and the Finnish Cultural Foundation Pirkanmaa Provincial Foundation.
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These authors contributed equally to this work.