Biochemical and Biophysical Research Communications
Apicidin is a histone deacetylase inhibitor with anti-invasive and anti-angiogenic potentials
Section snippets
Materials and methods
Materials. Apicidin, [cyclo(N-O-methyl-l-tryptophanyl-l-isoleucinyl-d-pipecolinyl-l-2-amino-8-oxodecanoyl)], was prepared from Fusarium sp. Strain KCTC 16677 according to the method previously described [14] and resuspended in dimethyl sulfoxide (DMSO). All other chemicals were of the highest quality commercially available.
Cells and cell culture. The v-ras-transformed mouse fibroblast NIH3T3 cells, human melanoma A2058 cells, and human breast cancer cells (MDA-MB-435s and MCF-7) were cultured
Results
In the previous study, to examine the effect of apicidin on the proliferation of mouse and human cancer cell lines, cell growth inhibition was assessed with the SRB protein dye assay 48 h after cell seeding at 1 × 105 cells/well in 6-well plates in complete growth medium [12]. In this assay, cell growth was inhibited to various degrees in the presence of apicidin, having half-maximum effects between 1.8 and 0.1 g/ml (IC50=0.18, 0.55, and 1.17 μg/ml in v-ras-NIH3T3, A2058, and MCF7, respectively).
Discussion
Since tumor metastasis is the process that requires for malignant cells to leave the primary tumor and proliferate at a distant site, this process is the leading cause of death in cancer patients. Tumor metastasis is characterized with several steps: invasion (matrix degradation and cell motility), intravasation, cell attachment, extravasation, cell proliferation, and vessel formation (angiogenesis) [17]. Recently, HDAC inhibitors are emerging as an exciting new class of potential anti-cancer
Acknowledgements
This work was supported by research Grant No. KPRC-99-KA-1-3 from Kyonggi Pharmaceutical Research Center.
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These authors contributed equally to this work and are equal first authors.