Biochemical and Biophysical Research Communications
BMP-2-induced Osterix expression is mediated by Dlx5 but is independent of Runx2
Section snippets
Materials and methods
Materials. Bioactive recombinant human BMP-2 (rhBMP-2) was from Genetics Institute (Cambridge, MA) and recombinant human TGF-β1 was purchased from R&D Systems (Minneapolis, MN). Cycloheximide was purchased from Sigma Chemical (St. Louis, MO). Superscript first-strand synthesis system for reverse transcription and Lipofectamine plus were from Invitrogen (Carlsbad, CA). Taq polymerase, dNTP mixture, and G418 were from Promega (Madison, WI). The Megaprime DNA labeling system kit was from
BMP-2 stimulates Osx expression through a newly synthesized protein
We examined the expression patterns of Osx in osteoblastic lineage cells, ROS 17/2.8 and MC3T3-E1 cells, and in chondrogenic ATDC5 cells. In the absence of BMP-2 treatment, the basal Osx expression was very strong in osteogenic ROS 17/2.8 and chondrogenic ATDC5 cells, while it was lower in MC3T3-E1 (Fig. 1a). The expression was stimulated strongly by BMP-2 treatment but not by TGF-β1 (Fig. 1a). In ATDC5 cells, Osx expression was even suppressed by TGF-β1. Unlike these osteogenic cells, basal
Acknowledgements
This study was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (01-PJ1-PG1-01CH08-0001 and 01-PJ3-PG6-01GN11-0002).
References (26)
- et al.
A kinase domain-truncated type I receptor blocks bone morphogenetic protein-2-induced signal transduction in C2C12 myoblasts
J. Biol. Chem.
(1997) - et al.
Constitutively active BMP type I receptors transduce BMP-2 signals without the ligand in C2C12 myoblasts
Exp. Cell. Res.
(1997) - et al.
Smad1 and smad5 act downstream of intracellular signalings of BMP-2 that inhibits myogenic differentiation and induces osteoblast differentiation in C2C12 myoblasts
Biochem. Biophys. Res. Commun.
(1997) - et al.
Smad5 and DPC4 are key molecules in mediating BMP-2-induced osteoblastic differentiation of the pluripotent mesenchymal precursor cell line C2C12
J. Biol. Chem.
(1998) - et al.
A BMP-inducible gene, Dlx5, regulates osteoblast differentiation and mesoderm induction
Dev. Biol.
(1999) - et al.
The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation
Cell
(2002) - et al.
Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation
Cell
(1997) - et al.
Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts
Cell
(1997) - et al.
The missing bone
Cell
(1997) - et al.
The protein kinase C pathway plays a central role in the fibroblast growth factor-stimulated expression and transactivation activity of Runx2
J. Biol. Chem.
(2003)
Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage
J. Cell. Biol.
TGF-beta signalling from cell membrane to nucleus through SMAD proteins
Nature
Differential regulation of Dlx gene expression by a BMP morphogenetic gradient
Int. J. Dev. Biol.
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