Arthroscopy: The Journal of Arthroscopic & Related Surgery
Original ArticleThe Effects of Fibroblast Growth Factor-2 on Rotator Cuff Reconstruction With Acellular Dermal Matrix Grafts
Section snippets
Methods
This investigation was approved by the Animal Studies Committee of our institution and by the Institutional Animal Care and Use Committee.
Adult male Sprague-Dawley rats (mean weight, 501 g; SD, 40 g) were assigned to 2 groups of 15 rats each. The controls were 5 unoperated age- and weight-matched male Sprague-Dawley rats; they were sacrificed to obtain 3 histologic and 7 biomechanical control shoulder specimens. In all operated rats, a large rotator cuff defect measuring 3 × 5 mm was created on
Gross examination
The surgical site exhibited no signs of infection or wound dehiscence in any of the rats. Scar tissue was noted around the acromioclavicular joint, but there were no adhesions or contractures limiting the shoulder range of motion. At each time point, the defect was bridged by tissue in all specimens from both the FGF-treated and FGF-untreated groups. Macroscopic evaluation of specimens from both groups obtained at 6 and 12 weeks showed a tendon-like structure; it was similar in appearance to
Discussion
This study showed that the locally applied FGF-2 improved the strength and maturity of the regenerated tendon in Sprague-Dawley rats with rotator cuff defects reconstructed with GraftJacket. At 2 weeks after surgery, the FGF-treated and untreated specimens exhibited similar tendon maturity scores and strength. At 6 and 12 weeks, the FGF-treated group showed significantly higher scores and strength than the untreated group.
The management of large or massive rotator cuff tears presents a
Conclusions
The remodeling of ADM grafts placed in rat rotator cuff tendon defects was accelerated by the local administration of FGF-2.
Acknowledgment
The authors acknowledge the help of the Center for Animal Resources and Development of our institution. They also thank Kaken Pharmaceutical and Wright Medical Technology for providing the FGF-2 and GraftJacket ADM, respectively.
References (34)
- et al.
The effect of cartilage-derived morphogenetic protein 2 on initial healing of a rotator cuff defect in a rat model
J Shoulder Elbow Surg
(2007) - et al.
Evolution of the Fgf and Fgfr gene families
Trends Genet
(2004) - et al.
Molecular and biological characterization of fibroblast growth factor, an angiogenic factor which also controls the proliferation and differentiation of mesoderm and neuroectoderm derived cells
Cell Differ
(1986) - et al.
A comparison of arthroscopic and open rotator cuff repair
Arthroscopy
(2005) - et al.
Rotator cuff repair using an acellular dermal matrix graft: An in vivo study in a canine model
Arthroscopy
(2006) - et al.
Rotator cuff regeneration using chitin fabric as an acellular matrix
J Shoulder Elbow Surg
(2006) - et al.
Reconstruction of large rotator cuff tendon defects with porcine small intestinal submucosa in an animal model
J Shoulder Elbow Surg
(2006) - et al.
Experimental fascial autografting for the supraspinatus tendon defect: Remodeling process of the grafted fascia and the insertion into bone
J Shoulder Elbow Surg
(2002) - et al.
Development and use of an animal model for investigations on rotator cuff disease
J Shoulder Elbow Surg
(1996) - et al.
Rotator cuff defect healing: A biomechanical and histologic analysis in an animal model
J Shoulder Elbow Surg
(1998)
Overuse activity injures the supraspinatus tendon in an animal model: A histologic and biomechanical study
J Shoulder Elbow Surg
The effect of fibrin clot on healing rat supraspinatus tendon defects
J Shoulder Elbow Surg
The localized expression of extracellular matrix components in healing tendon insertion sites: An in situ hybridization study
J Orthop Res
Biologic augmentation of rotator cuff tendon-healing with use of a mixture of osteoinductive growth factors
J Bone Joint Surg Am
Effects of basic fibroblast growth factor on bone formation in vitro
J Clin Invest
Single local injection of recombinant fibroblast growth factor-2 stimulates healing of segmental bone defects in rabbits
J Orthop Res
Healing of full-thickness defects of the articular cartilage in rabbits using fibroblast growth factor-2 and a fibrin sealant
J Bone Joint Surg Br
Cited by (79)
Fibroblast Growth Factor Soaked Collagen Membrane Shows No Biomechanical or Histological Advantages in the Treatment of Chronic Rotator Cuff Tears in a Rabbit Model
2024, Arthroscopy - Journal of Arthroscopic and Related SurgeryAn engineered tenogenic patch for the treatment of rotator cuff tear
2022, Materials and DesignCitation Excerpt :These tissue sections were stained with hematoxylin and eosin (HE), Masson, picrosirius red and Safranin O/fast green. HE staining results were analyzed by the modified Watkin score [26] and the semi-quantitative scoring system [27]. The picrosirius red staining by polarized light microscopy can distinguish collagen type I (yellow) or type III (green).
3D bioprinting of multilayered scaffolds with spatially differentiated ADMSCs for rotator cuff tendon-to-bone interface regeneration
2022, Applied Materials TodayCitation Excerpt :Sections were then stained with H&E staining, Masson's trichrome (MT) staining, Safranin O staining, and picrosirius red (PR) staining. The healing of the reconstructed infraspinatus enthesis was assessed via a tendon maturing scoring system as previously described by Ide et al. (Table S2) [52]. The images were blindly examined and scored by two researchers.
Evaluation of patches for rotator cuff repair: A systematic review and meta-analysis based on animal studies
2022, Bioactive MaterialsCitation Excerpt :Liu, G. M [97] found that there was no significant difference between multi-layer acellular tendon sheet and autologous tendon. For acellular dermal matrix grafts, two studies [77,95] added factors to the grafts, and the results of the group adding factors were better than those of the simple suture group. Adams, J. E [41] found there was no significant difference between human acellular dermal matrix and autologous tendon.
Biologics in rotator cuff repair
2022, Surgical Techniques of the Shoulder, Elbow, and Knee in Sports Medicine, Third Edition
Supported by a 2006 grant from the Japan Sports Medicine Foundation. The authors report no conflict of interest.