The effect of fat content on the rheology, microstructure and heat-induced functional characteristics of Cheddar cheese

https://doi.org/10.1016/S0958-6946(00)00048-0Get rights and content

Abstract

Cheddar cheeses with the different fat contents were made in triplicate and ripened at 4°C for 30 d and at 7°C for the remainder of the 180-d investigation period. The cheeses were designated: full-fat (FFC), 300 g kg−1; reduced-fat (RFC), 219 g kg−1; half-fat (HFC), 172 g kg−1; and low-fat (LFC), 71.5 g kg−1. A decrease in the fat content from 300 to ≤172 g kg−1 resulted in significant (P<0.05) decreases in contents of moisture in non-fat substance and pH 4.6 soluble N (expressed as % total N), and increases in the contents of moisture, protein, intact casein and free amino acids. Reduction in fat content resulted in an increase in the volume fraction of the casein matrix and a decrease in the extent of fat globule clumping and coalescence. The mean values of fracture stress and firmness for the FFC were significantly lower than those of the RFC and HFC, which had similar values; the values for the LFC exceeded the limits of the test and were markedly higher than those of the other cheeses at all times. On baking the cheese, reduction in fat content resulted in significant increases in the mean melt time (time required for shred fusion) and apparent viscosity and a decrease in the mean flowability of the melted cheese. The stretchability of the FFC increased most rapidly and, at ∼15 and 30 d, attaining mean values which were significantly higher than those of the other cheeses. Thereafter the stretchability of the FFC decreased progressively to values that were significantly (i.e. at 150 d) or numerically (i.e., at 180 d) lower than those of the RFC and HFC. At ripening times ≥15 and ≤90 d, the stretchability of the LFC was significantly lower than that of the RFC, and significantly or numerically lower than the HFC.

Introduction

Recent market research suggests that reduction in fat content and delivery of desirable functionality are two of the major approaches by which international cheese consumption can be increased (Market Tracking International, 1998). Hence, many studies have investigated the effects of various treatments on the proteolysis, texture and microstructure of reduced-fat cheeses with a view to improving quality (Ardö, Larsson, Mansson & Hedenberg, 1989; Banks, Brechany & Christie, 1989; Tunick et al., 1993; Kucukoner & Haque, 1995; Bryant, Ustanol & Steffe, 1995; Desai & Nolting, 1995; Drake, 1997; Fenelon & Guinee, 1997; Guinee et al., 1998a). Comparatively few studies have investigated the heat-related functionality of reduced-fat cheese and the factors affecting it (Tunick, Mackey, Smith & Holsinger, 1991; Merrill, Oberg & McMahon, 1994; Tunick, Malin, Smith & Holsinger, 1995; McMahon, Alleyne, Fife & Oberg, 1996; Rudan, Barbano, Guo & Kindstedt, 1998a, Rudan, Barbano & Kindstedt, 1998b; Poduval & Mistry, 1999); the latter studies pertain mostly to Mozzarella. Furthermore, the effect of reducing fat content (e.g., from full-fat to half-fat) on the microstructure, proteolysis, texture and/or functionality of cheese has received little attention (Mistry & Anderson, 1993; Bryant et al., 1995; Mackey & Desai, 1995; Tunick & Shieh, 1995; Rudan et al., 1998a, Rudan et al., 1998b). In particular, little has been reported on the effect of incremental fat reduction on cheese functionality (Fife, McMahon & Oberg, 1996; Rudan & Barbano; 1998; Rudan, Barbano, Yun & Kindstedt, 1999), especially for cheeses other than Mozzarella, e.g., Cheddar (Olson & Bogenrief, 1995). The limited comparison between full- and half-fat Mozzarella has shown that fat reduction results in an increase in firmness and a decrease in the quantity of free oil exuded on baking and flowability.

The objective of the current study was to investigate the effects of incremental fat reduction, in the range 71–300 g kg−1, on the composition, rheology, microstructure and functionality of Cheddar cheese.

Section snippets

Standardization of milks

Raw milk was obtained from a local dairy company. Part of the milk was separated at 55°C and the resultant skim milk was added to whole milk to give standardized milks with casein to fat ratios ranging from 4.8 to 0.75 (Table 1). These milks were held overnight at 4°C, pasteurized at 72°C×15 s, cooled to 31°C, pumped to the cheese vat, and converted to Cheddar cheeses of different fat levels (Table 2), arbitrarily designated as: full-fat (∼300 g kg−1; FFC); reduced fat (∼219 g kg−1; RFC); half-fat

Milk composition

The compositions of the standardised pasteurized milks from the three replicate trials are given in Table 1. Increasing the milk fat level was paralleled by a small reduction in the casein content, with the mean level in the full-fat milk being significantly lower than that in the low-fat milk. In contrast, the reduction in protein content was not significant.

Cheese composition

Reduction in fat level resulted in significant (P<0.05) increases in the contents of moisture (0.37 g g−1 fat), protein (0.52 g g−1 fat), ash

Conclusions

The study indicated that fat has a major effect on the microstructure, texture and functionality of Cheddar cheese. The fracture stress and firmness increased as the fat level was decreased in the range 300–71.5 g kg−1. Reduction in fat content resulted in increases in the apparent viscosity and melt time and a decrease in the flowability of the baked cheese at most ripening times over the 180 d ripening period. At times ≤64 d the stretchability of the baked cheese decreased significantly with fat

Acknowledgements

This project was part-funded by the European Union Structural Funds (European Regional Development Fund). The authors kindly acknowledge the technical assistance of C. Mullins, M.O. Corcoran and E.O. Mulholland.

References (72)

  • M.A Rudan et al.

    Effect of fat reduction on chemical composition, proteolysis, functionality, and yield of Mozzarella cheese

    Journal of Dairy Science

    (1999)
  • M.H Tunick et al.

    Effects of skim milk homogenization on proteolysis and rheology of Mozzarella cheese

    International Dairy Journal

    (1995)
  • M.H Tunick et al.

    Proteolysis and rheology of reduced-fat Mozzarella cheese prepared from homogenized milk

    Journal of Dairy Science

    (1993)
  • Y Ardö et al.

    Studies of peptidolysis during early maturation and its influence on low-fat cheese quality

    Milchwissenschaft

    (1989)
  • A.G Altemueller et al.

    Monitoring proteolysis during ripening of full-fat and low-fat Cheddar cheese by reverse-phase HPLC

    Journal of Food Science

    (1996)
  • J.M Banks et al.

    The production of low-fat Cheddar cheese

    Journal of the Society of Dairy Technology

    (1989)
  • D.D Bogenrief et al.

    Hydrolysis of β-casein increases Cheddar cheese meltability

    Milchwissenschaft

    (1995)
  • R Boutrou et al.

    Changes in the composition of juice expressed from Camembert cheese during ripening

    Lait

    (1999)
  • British Standards Institution (1975). Chemical analysis of cheese. Part 5. Determination of pH value. British Standard...
  • A Bryant et al.

    Texture of Cheddar cheese as influenced by fat reduction

    Journal of Food Science

    (1995)
  • Creamer, L. K. (1971). β-Casein hydrolysis in Cheddar cheese ripening. New Zealand Journal of Dairy Science and...
  • L.K Creamer et al.

    Rheological evaluation of maturing Cheddar cheese

    Journal of Food Science

    (1982)
  • L de Jong

    Protein breakdown in soft cheese and its relation to consistency. 2. The influence of rennet concentration

    Netherlands Milk and Dairy Journal

    (1977)
  • de Jong, L (1978). The influence of moisture content on the consistency and protein breakdown of cheese. Netherlands...
  • Desai, N., & Nolting, J. (1995). Microstructure studies of reduced fat cheeses containing fat substitute. In: E. L....
  • M.A Drake

    Rheology of full-fat and low-fat Cheddar cheese as related to type of fat mimetic

    Journal of Food Science

    (1997)
  • M.A Fenelon et al.

    The compositional, textural and maturation characteristics of reduced-fat Cheddar made from milk containing added Dairy-LoTM

    Milchwissenschaft

    (1997)
  • L.F Fife et al.

    Functionality of low-fat Mozzarella cheese

    Journal of Dairy Science

    (1996)
  • T.J Geurts et al.

    Water binding to milk protein, with particular reference to cheese

    Netherlands Milk and Dairy Journal

    (1974)
  • J Gilles et al.

    The yield of cheese

    New Zealand Journal of Dairy Science and Technology

    (1985)
  • T.P Guinee et al.

    Effect of milk protein standardization, by ultrafiltration, on the manufacture, composition and maturation of Cheddar cheese

    Journal of Dairy Research

    (1994)
  • T.P Guinee et al.

    Observations on the microstructure and heat-induced changes in the viscoelasticity of commercial cheeses

    Australian Journal of Dairy Technology

    (1999)
  • T.P Guinee et al.

    The influence of milk pasteurization temperature and pH at curd milling on the composition, texture and maturation of reduced fat Cheddar cheese

    International Journal of Dairy Technology

    (1998)
  • Guinee, T. P., Mulholland, E. O., Mullins, C., & Corcoran, M. O. (1997). In: T. M. Cogan, P. F. Fox & P. Ross,...
  • T.P Guinee et al.

    Effect of altering the daily herbage allowance to cows in mid lactation on the composition, ripening and functionality of low-moisture, part-skim Mozzarella cheese

    Journal of Dairy Research

    (1998)
  • Horne, D. S., Banks, J. M., Leaver, J., & Law, A. J. R. (1994). Dynamic mechanical spectroscopy of Cheddar cheese. In...
  • Cited by (187)

    • Melting of natural cheese: A review

      2023, International Dairy Journal
    View all citing articles on Scopus
    View full text