Elsevier

Neuroscience Letters

Volume 314, Issue 3, 16 November 2001, Pages 115-118
Neuroscience Letters

The levels of soluble amyloid beta in different high density lipoprotein subfractions distinguish Alzheimer's and normal aging cerebrospinal fluid: implication for brain cholesterol pathology?

https://doi.org/10.1016/S0304-3940(01)02263-7Get rights and content

Abstract

Several previous studies reported the association of the soluble form of amyloid β (sAβ) protein, a major constituent of amyloid deposits in Alzheimer's disease (AD), with normal blood, cerebrospinal fluid (CSF) and central nervous system high density lipoproteins (HDLs). The present report aimed to elucidate the pattern of sAβ and apolipoprotein (apo) distribution in AD CSF-HDL subfractions. We studied AD CSF-HDL subfractions by SDS/PAGE and immunoblot analysis after CSF fractionation via density flotation ultracentrifugation. AD CSF was characterized by (i) increased sAβ and apo content of the HDL1, and (ii) sAβ association with apoE and apoJ in HDL2, HDL3 and very high density lipoproteins. The finding supports our proposed hypothesis that upregulation of brain cholesterol dynamics is a fundamental event in the pathophysiology of AD and that sAβ binding to apo and lipid may have important structure-functional consequences.

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Acknowledgements

This research was supported in part by Russian Academy of Medical Sciences and Senetek, PLC. The preliminary account of this paper was presented at the Society for Neuroscience (USA) Annual Meeting, New Orleans, LA, USA, on November 5, 2000.

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