Cell
Volume 62, Issue 3, 10 August 1990, Pages 481-492
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Article
Association between the PDGF receptor and members of the src family of tyrosine kinases

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Abstract

We have examined the interaction between the plate-let-derived growth factor (PDGF) receptor and three src family tyrosine kinases, pp60c-src, p59fyn, and pp62c-yes. The kinase activities of all three enzymes were elevated after PDGF stimulation of quiescent fibroblasts, coincident with association of the src family kinases with the PDGF receptor and other proteins. The presence of a protein of 81–85 kd in these complexes correlated with the detection of phosphatidylinositol (PI) kinase activity (previously described to associate with both the PDGF receptor and pp60c-src-middle T antigen). These results suggest that the physiological response to PDGF involves interaction of the receptor not only with serine/threonine and lipid kinases and a phospholipase, but also with other tyrosine kinases.

References (40)

  • D.K. Morrison et al.

    Direct activation of the serine/threonine kinase activity of Raf-1 through tyrosine phosphorylation by the PDGF β-receptor

    Cell

    (1989)
  • A.S. Shaw et al.

    The lck tyrosine protein kinase interacts with the cytoplasmic tail of the CD4 glycoprotein through its unique aminoterminal domain

    Cell

    (1989)
  • A. Veillette et al.

    The CD4 and CD8 T cell surface antigens are associated with the internal membrane tyrosine-protein kinase p56lck

    Cell

    (1988)
  • S. Bishayee et al.

    Characterisation of a novel anti-peptide antibody that recognises a specific conformation of the platelet-derived growth factor receptor

    Mol. Cell. Biol.

    (1988)
  • S.H. Cheng et al.

    Peptide antibodies to the human c-fyn gene product demonstrate p59fyn is capable of complex formation with the middle-T antigen of polyomavirus

    EMBO J.

    (1988)
  • J.A. Cooper

    The src-family of protein-tyrosine kinases

  • J.A. Cooper

    Activation of pp60c-src kinase by middle-T antigen binding or by dephosphorylation

    EMBO J.

    (1985)
  • S.A. Courtneidge et al.

    Polyoma virus transforming protein associates with the product of the c-src gene

    Nature

    (1983)
  • S.A. Courtneidge et al.

    The complex of polyoma virus middle-T antigen and pp60c-src

    EMBO J.

    (1984)
  • C.E. Ellis et al.

    Phosphorylation of GAP and GAP-associated proteins by transforming and mitogenic tyrosine kinases

    Nature

    (1990)
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