Immobilization stress-induced antioxidant defense changes in rat plasma: Effect of treatment with reduced glutathione

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Abstract

  • 1.

    1. We examined immobilization stress-induced antioxidant defense changes in rat plasma and observed the antioxidant effect of reduced glutathione (GSH) administration on these changes.

  • 2.

    2. Immobilization stress induced severe bleeding in the stomach and a significant increase in plasma levels of thiobarbituric acid receives substances (TBARS).

  • 3.

    3. Immobilization stress induced a significant decrease in plasma iron-binding, ironoxidizing protections and radical scavenging activity.

  • 4.

    4. Plasma levels of ascorbic acid, ascorbyl radical and superoxide dismutase activity remained unchanged following immobilization stress.

  • 5.

    5. Treatment with GSH showed a significant protective effect on stomach bleeding, on the increase in plasma TEARS, and on the decrease of iron-binding, iron-oxidizing protection and radical scavenging activity in plasma.

  • 6.

    6. These results suggest that immobilization stress induces generation of reactive oxygen species and decreases the endogenous antioxidant defenses, which can be attenuated by extracellular administration of antioxidant GSH.

References (39)

  • R. Munday et al.

    Reduced glutathione in combination with superoxide dismutase as an important biological antioxidant defense mechanism

    Biochem. Pharmacol.

    (1989)
  • H. Ohkawa et al.

    Assay for lipid peroxides in animal tissue by thiobarbiturie acid reaction

    Analyt. Biochem.

    (1979)
  • X. Wang et al.

    Direct detection of circulating free radicals in the rat using electron spin resonance spectrometry

    Free Radical Biol. Med.

    (1992)
  • B.S. Winkler

    In vitro oxidation of ascorbic acid and its prevention by GSH

    Biochim. Biophys. Acta

    (1987)
  • B.N. Ames et al.

    Uric acid provides an antioxidant defense in humans against oxidant- and radical-caused aging and cancer: a hypothesis

  • E. Culotta et al.

    NO news is good news

    Science

    (1992)
  • R.G. Cutler

    Antioxidants and aging

    Am. J. Clin. Nutr.

    (1991)
  • T.A. Deviatkina et al.

    Antioxidant deficiency and reaction of tissue on the acute emotional-pain stress

    Vopr. Med. Khim.

    (1989)
  • M.J. Davies

    Detection ofmyoglobin-derived radicals on reaction of metmyoglobin with hydrogen peroxide and other peroxidic compounds

    Free Radical Res. Commun.

    (1990)
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