Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology
EndodontologyOnline only articleReaction of rat connective tissue to a new calcium hydroxide–based sealer
Section snippets
Material and Methods
In this study, 36 4- to 6-month-old male Wistar albino rats weighing 250-280 g were used. The care of the animals was carried out according to Araçatuba School of Dentistry–UNESP Ethical Committee, which approved the project before the beginning of the experiments. The following materials were investigated:
Group 1: Acroseal (Septdont, Saint Maur des Fossés, France), lot no. P2 209. Composition according to the manufacturer: base: glycyrrhetic acid (enoxolone), methenamine, radiopaque excipient
Results
The dentin tubes which were demineralized were used to evaluate the inflammatory reaction, and that ones without demineralization were used to observe the presence or not of the mineralization stimulated or not by the materials. Scoring of the inflammatory reactions on the 7th and 30th day is given in Table I.
Discussion
Several methods have been used to evaluate the biocompatibility of endodontic materials, including subcutaneous implantation tests.14, 15 Implantation in subcutaneous tissue of rats is one of the most appropriate tests to determine the local effects of materials.11, 16, 17 In the present investigation, empty dentin tubes from control groups promoted few or no reaction in subcutaneous tissue and produced normal repair tissues, similar to results previously reported.9, 18
Tissue reaction to the
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Effects of fly ash and boric acid on Y<inf>2</inf>O<inf>3</inf>-stabilized tetragonal ZrO<inf>2</inf> dispersed with MgAl<inf>2</inf>O<inf>4</inf>: An experimental study on rat subcutaneous tissue
2015, Annals of AnatomyCitation Excerpt :Therefore, the present study has the limitation of not using the un-modified zirconia as control material. Additionally, in implantation studies, the traumatic injury due to experimental procedures and the surgical process of implantation might be responsible for the initially observed inflammatory reaction (Gomes-Filho et al., 2008). In addition to the zirconia insertions, a sham operation was performed on each rat in the control group.
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