Bone marrow and stem cell transplantationGalectin-3 Enhances Proliferation and Angiogenesis of Endothelial Cells Differentiated from Bone Marrow Mesenchymal Stem Cells
Section snippets
Antibodies and Reagents
LG-DMEM (Hyclone, San Diego, Calif), trypsin-EDTA solution (Beyotime, Jiangsa, China), fetal bovine serum (FBS; Sijiqing, China), percoll (Pharmacia, Kalamazoo, Mich), galectin-3 (Peprotechm Rocky HIll, NJ) vascular enthelial growth actor (VEGF; Peprotech), basic fibroblast growth factor (bFGF; Peprotech), polyclonal ntibody (Promega, Madiso, Wisc), rabbit–anti-mouse polyclonal antibodies against Von Willebrand factor (vWF; Promega), MTT (Amresco, Solon, Oh), propidine iodide (PI, Sigma, Louis,
Cell Culture
Cells were adhered in plastic flasks, P0 (passage zero) cells appeared in flasks after 2 days of plating with a fibroblast-like, spindle-shaped or polygonal morphology. These cells began to proliferate at about day 4 and gradually grew to form small colonies (Fig 1A). By day 7–8, the number of cellular colonies of different sizes had obviously increased. After approximately 12 days, cells became confluent (Fig 1B). The behavior of passaged MSC was similar to that in primary culture, 0 in the
Discussion
MSCs have shown all criteria of true stem cells, including self-renewal, multilineage differential, and reconstitution of tissue.10 The use of autologous vascular endothelial progenitor cells seems attractive to the development of engineered vessels as well as to the vascularization of engineered tissues, and may be useful for augment vessel growth in ishcemic tissue.11, 12 MSCs have potency of oriented differentiation into vessel endothelial cells under certain conditions and can be used in
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Cited by (33)
Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice: Novel implication for vascular atrophy
2018, Experimental Eye ResearchCitation Excerpt :In contrast to their inhibiting the blood supply, there is another possibility that galectin-3-expressing cells accumulate in the hyaloid vessels under ischemic conditions for neovasculogenesis. Galectin-3 induces the differentiation of endothelial cells in vitro and stimulates angiogenesis in vivo (Nangia-Makker et al., 2000; Wan et al., 2011). The angiogenetic effects of galectin-3 contradict our conclusion showing the involvement of galectin-3 in vascular atrophy.
The emerging role of galectins in cardiovascular disease
2016, Vascular PharmacologyCitation Excerpt :Wan et al. investigated the effect of galectin-3 on endothelial cells differentiated from rat bone marrow mesenchymal stem cells. They found that galectin-3 enhances endothelial cell proliferation and tube formation, associated with angiogenesis [41]. Pro-angiogenic effects of galectin-3 can also be mediated by an indirect effect via circulating platelets.
Galectins in angiogenesis: Consequences for gestation
2015, Journal of Reproductive ImmunologyCitation Excerpt :Thus, a possibility that awaits further investigation is the involvement of this lectin in the modulation of trophoblast differentiation during placentation, particularly in view of recent studies showing a significant up-regulation of gal-3 protein upon hypoxia-induced syncytialisation in the BeWo cell line (Hu et al., 2007). Additionally, several studies have attributed pro-angiogenic properties to this lectin (Nangia-Makker et al., 2000b; Fukushi et al., 2004; Wan et al., 2011; Machado et al., 2014), which are anticipated to be significant for vascular responses associated with pregnancy and are discussed in detail in the following sections. As for tandem repeat galectins, the only members identified to date at the foetal–maternal interface include gal-8, gal-9 and gal-4, although the latter has only been described in rat placenta and scant information on its physiological role is available (Arikawa et al., 2012).
The impact of galectin-3 inhibition on aldosterone-induced cardiac and renal injuries
2015, JACC: Heart FailureBlocked angiogenesis in Galectin-3 null mice does not alter cellular and behavioral recovery after middle cerebral artery occlusion stroke
2014, Neurobiology of DiseaseCitation Excerpt :Gal-3 exacerbates inflammation and striatal damage in neonatal hypoxia/ischemia (Doverhag et al., 2010). Other studies show that it stimulates endothelial cell proliferation and capillary vessel morphogenesis (Nangia-Makker et al., 2000; Wan et al., 2011). Gal-3 is generally only expressed in the central nervous system after brain injury (Mok et al., 2007; Walther et al., 2000), however we found it in the healthy SVZ stem cell niche (Comte et al., 2011).
Vascular galectins: Regulators of tumor progression and targets for cancer therapy
2013, Cytokine and Growth Factor ReviewsCitation Excerpt :These effects appear to be concentration dependent as higher concentrations can reduce endothelial function [26] and even induce apoptosis [71]. Angiostimulatory activities have also been attributed to galectin-3 [69,72–76]. Markowska and co-workers showed that galectin-3 induces a concentration dependent angiogenic response in vivo using the mouse corneal micropocket assay [73] while Nangia-Makker et al. showed that galectin-3 acts as a chemo-attractant for endothelial cells [74,77] thereby stimulating in vivo vascularization [74].
S.Y. Wan and T.F. Zhang contributed equally to this work.
Supported in part by the Central Research Laboratory of the First Affiliated of Anhui Medical University and Department of Health of Anhui Province fund.