Editorial

Potential hazard of nanoparticles: From properties to biological and environmental effects

This review aims to systematize data on the construction and applications of bacterial lux-biosensors in various fields ranging from investigation of gene regulation and regulatory networks to the new probiotics' search and ecotoxicological research. The typical technical solutions and devices required for diverse tasks applying lux-biosensors are reviewed. Aspects of the application of lux-biosensors in fundamental researches, such as the study of oxidative stress, heat shock, DNA-damaging, pro- and antioxidant activities, are also considered. This technology allows rapid screening of the biological activities of newly synthesized compounds, which could be applied as components for fuels, household chemicals, and drugs. Works related to the ecological state assessment on water resources are also described. The use of lux-biosensor complexes based on different organisms, including both gram-positive and gram-negative bacteria, makes toxicological investigations more comprehensive. Bacterial lux-biosensors based on Escherichia coli can be used as a model for evaluation of the effect of certain substances on the transmembrane potential in mitochondria, albeit with extrapolation to a certain extent. Another aspect that draws our interest is that biosensors are able to help predict some systemic properties of probiotics. In the future, it's quite promising to see more applications of lux-biosensors for environmental control, microbial-microbial interaction assessment, antioxidant action mechanism studies and toxicological studies in the development of new drugs.

The current review aims to gain knowledge on the biosynthesis and characterization of nanoparticles (NPs), their multifactorial role, and emerging trends of NPs utilization in modern science, particularly in sustainable agriculture, for increased yield to solve the food problem in the coming era. However, it is well known that an environment-friendly resource is in excessive demand, and green chemistry is an advanced and rising resource in exploring eco-friendly processes. Plant extracts or other resources can be utilized to synthesize different types of NPS. Hence NPs can be synthesized by organic or inorganic molecules. Inorganic molecules are hydrophilic, biocompatible, and highly steady compared to organic types. NPs occur in numerous chemical conformations ranging from amphiphilic molecules to metal oxides, from artificial polymers to bulky biomolecules. NPs structures can be examined by different approaches, i.e., Raman spectroscopy, optical spectroscopy, X-ray fluorescence, and solid-state NMR. Nano-agrochemical is a unification of nanotechnology and agro-chemicals, which has brought about the manufacture of nano-fertilizers, nano-pesticides, nano-herbicides, nano-insecticides, and nano-fungicides. NPs can also be utilized as an antimicrobial solution, but the mode of action for antibacterial NPs is poorly understood. Presently known mechanisms comprise the induction of oxidative stress, the release of metal ions, and non-oxidative stress. Multiple modes of action towards microbes would be needed in a similar bacterial cell for antibacterial resistance to develop. Finally, we visualize multidisciplinary cooperative methods will be essential to fill the information gap in nano-agrochemicals and drive toward the usage of green NPs in agriculture and plant science study.

Oxidative stress (root cell viability, hydrogen peroxide and malondialdehyde) and subsequent antioxidant enzyme responses (ascorbate peroxidase, total superoxide dismutase, glutathione reductase and glutathione-S-transferase) and apoptotic cell death (using flow cytometric analysis) are studied following the application of NPs (Cu and CdS) in a plant based system (Nigella sativa L., Family-Ranunculaceae). The objective of the work is to assess the oxidative damaging potentiality of NPs in a host system for their suitable applicability. The present investigation highlights the following: 1) chemogenic synthesis of metallic class Cu- and semiconductor class CdS-NPs exhibit nanodimension range miniaturization of the atomic aggregates which readily applicable for nanocrystal-plant interaction, 2) uptake of Cu- and CdS-NPs in seedlings shows particle sizing effect, 3) differential stress responsiveness as evinced from H₂O₂ generation and MDA accumulation, 4) disproportional activation of tSOD – insignificant elevation in Cu-NPs contrasting to pronounced activation in CdS-NPs, 5) non-synchronous activation of GR-GST coupled system suggesting suppressivity in their orderly functioning, 6) Evans blue dye uptake test as well as flow cytometric study demonstrate enhancement in cell mortality through NPs induced apoptosis and 7) cell cycle analyses also suggest G₁ phase blockage and cellular metabolic peak shifting towards early G₁ phase.

Nanometer-sized structures, surfaces and sub-surface phenomena have played an enormous role in science and technological applications and represent a driving-force of current interdisciplinary science. Recent developments include the atomic-scale characterization of nanoparticles, molecular reactions at surfaces, magnetism at the atomic scale, photoelectric characterization of nanostructures as well as two-dimensional solids. Research and development of smart nanostructured materials governed by their surface properties is a rapidly growing field. The main challenge is to develop an accurate and robust electronic structure description. The density of surface-related trap states is analyzed by transient UV photoconductivity and temperature-dependent admittance spectroscopy. An advanced application of thin films on shaped substrates is the deposition of catalytic layers on hollow glass microspheres for hydrogen storage controlled exothermal hydrolytic release. Surface properties of thin films including dissolution and corrosion, fouling resistance, and hydrophilicity/hydrophobicity are explored to improve materials response in biological environments and medicine. Trends in surface biofunctionalization routes based on vacuum techniques, together with advances in surface analysis of biomaterials, are discussed. Pioneering advances in the application of X-ray nanodiffraction of thin film cross-sections for characterizing nanostructure and local strain including in-situ experiments during nanoindentation are described. Precise measurements and control of plasma properties are important for fundamental investigations and the development of next generation plasma-based technologies. Critical control parameters are the flux and energy distribution of incident ions at reactive surfaces; it is also crucial to control the dynamics of electrons initiating non-equilibrium chemical reactions. The most promising approach involves the exploitation of complementary advantages in direct measurements combined with specifically designed numerical simulations. Exciting new developments in vacuum science and technology have focused on forward-looking and next generation standards and sensors that take advantage of photonics based measurements. These measurements are inherently fast, frequency based, easily transferrable to sensors based on photonics and hold promise of being disruptive and transformative. Realization of Pascal, the SI unit for pressure, a cold-atom trap based ultra-high and extreme high vacuum (UHV and XHV) standard, dynamic pressure measurements and a photonic based thermometer are three key examples that are presented.

Nanotechnology is comprised of a wide range of technologies dealing with nano-sized structures (at least one dimension from 1 to 100 nm). Nanomaterials are classified as naturally occurring, incidentally synthesized, and intentionally manufactured. Potential benefits of manufactured nanoparticles (NPs) with different chemical compositions have been welcomed in various parts of the food industry such as food packaging and food security. Oral administration is the main way of introducing NPs into the human body. It has been demonstrated by the researchers that NPs have the potential to pose a greater threat to microbiota than bulk materials due to their particle size, surface chemistry, shape, internal structure, and intermediate stability. Even though nanotechnology is now widely applied in many areas of the food industry, the potential risks their extensive use could pose to the consumers and manufactured products are not yet fully understood. The literature shows that there is a knowledge gap about the presence or amounts of different NPs that could enter human body through different foodstuffs. This article is a review of the ways that the most widely found NPs enter human body, animals, plants and the environment. It focuses on the potential biosafety problems NPs might cause upon entering the human body. It also discusses the factors involved in the toxicity of NPs.

Copper nanoparticles (CuNPs) are used widely due to their attractive antimicrobial properties. However, their biosafety and kinetics on vertebrate embryogenesis are still limited. In this study, CuNPs were revealed to induce eye hypoplasia and almost no digestive gut in zebrafish embryos in a dose-dependent manner. Then, transcriptional responses of zebrafish embryos to CuNPs were investigated, and it was revealed that the genes related to wound healing and stimulus responses were up-regulated, but the genes associated with phototransduction and metabolisms were down-regulated. Differentially expressed genes (DEGs) in CuNPs-exposed and Cu²⁺-exposed embryos were compared further. Increased VEGF signaling and expression of fli1 were observed in CuNPs rather than Cu²⁺ treated embryos, but increased reactive oxygen species (ROS) and the resulting enhanced hemoglobin were observed in both CuNPs and Cu²⁺ treated embryos. This study for the first time revealed that CuNPs and Cu²⁺ both down-regulated the genes related to phototransduction and metabolisms, but up-regulated the genes associated with hemoglobin. Additionally, compared with Cu²⁺, CuNPs might be more effective in elevating blood vessels in embryos. Our results suggest that the biological effects of CuNPs are organogenesis-specific during fish embryogenesis, and both particles and ions might mediate their biological effects on embryogenesis.