Research ArticleImaging of size-dependent uptake and identification of novel pathways in mouse Peyer's patches using fluorescent organosilica particles
Graphical Abstract
Single-particle and simultaneous dual-particle administration of fluorescent organosilica particles revealed size-dependent uptake and presents novel pathways in mouse Peyer’s patches.
Section snippets
Materials
Rhodamine B, 3-mercaptopropyltrimethoxysilane (MPMS), (3-Aminopropyl)trimethoxysilane (APS), fluorescein isothiocyanate (FITC) and the lectin Ulex europaeus agglutinin I (UEAI) conjugated to FITC were purchased from Sigma–Aldrich Chemical Co. (Saint Louis, Missouri). Anti-mouse CD11b (Mac 1 α chain) antibody and FITC mouse anti-rat IgG secondary antibody was purchased from eBioscience, Inc., (Kobe, Japan). Paraformaldehyde was purchased from Merck KGaA (Darmstadt, Germany). Osmium tetroxide was
Size and time dependency of fluorescent thiol-organosilica particles uptake by mouse PPs
Figure 2 shows the FM of mouse PPs 1, 3 and 6 hours after oral administration of various sizes of particles containing rhodamine B. The fluorescence from Rh95, Rh130 and Rh200 in the subepithelial dome was increased at 6 hours in comparison with those at 1 and 3 hours. For both Rh340 and Rh695, the fluorescence in the subepithelial dome after 1 and 3 hours was almost similar, but after 6 hours the fluorescence increased. Very little fluorescence was observed in the subepithelial dome for
Discussion
In this study, we orally administered various sizes of fluorescent thiol-organosilica particles to study the size-dependent uptake through PPs. The particles could be taken up by PPs in a way that is similar to that of polystyrene,15, 23, 39 chitosan,4 poly DL-lactide-co-glycolide (PLAGA),40 titanium dioxide particles41 and other particles.19, 22,24, 25, 26, 27, 28, 29 Several physical properties of the NPs can influence their interaction within the M cells and other cells.14, 15, 16, 17, 18 If
Acknowledgments
This work was supported partly by a Grant-in-Aid for Younger Scientists (to M. Nakamura), by a Grant for Practical Application of University Research and Development Results under the Matching Fund Method (to M. Nakamura) from the New Energy and Industrial Technology Development Organization (NEDO) of Japan by a Grant-in-Aid for Scientific Research (C) (to M. Nakamura), and by the financial support of the Egyptian Ministry of Higher Education and Scientific Research, Missions Sector (to A.
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Cited by (0)
We thank the Egyptian Ministry of Higher Education and Scientific Research, Missions Sector for supporting the author (Aziz Awaad).