Clinical ResearchMicroRNA Expression Profiles in External Cervical Resorption
Section snippets
Study Participants and Sample Collection
This was a single-center pilot study that evaluated the miRNA levels in individuals with ECR or CP. Granulomatous tissue (test) samples and clinically asymptomatic or clinically healthy gingival biopsy (control) samples were collected from participants with ECR or CP at the time of the surgical treatment. In total, 8 participants (5 with ECR and 3 with CP) were recruited from a pool of individuals seeking dental treatment at the University of North Carolina at Chapel Hill School of Dentistry’s
Results
Demographic, clinical, and pulp diagnosis data of the study participants are shown in Supplemental Table S1 (available online at www.jendodon.com). All ECR-involved teeth were either maxillary or mandibular anterior teeth. The teeth with ECR, having had a history of trauma, all had the diagnosis of necrotic pulp. Interestingly, the cases of unknown etiology for ECR had vital pulp status. Figure 1 depicts radiographic evidence of 2 ECR-involved teeth (#6 and #24) from different patients. ECR
Discussion
In this report, we sought to uncover the potential role of miRNAs in ECR and CP, both of which are inflammatory diseases that possess unique pathogenicity. Clinically, ECR and CP have a common feature regarding the resorption of the adjacent hard tissues in which ECR affects the cementum and dentin and CP affects the alveolar bone. To our knowledge, this pilot study is the first to identify differential expression of miRNAs in ECR. By comparing the differentially regulated miRNA species in ECR
Acknowledgments
Supported in part by the American Association of Endodontists Foundation and the National Institutes of Health/National Institute of Dental and Craniofacial Research (grant nos. R21 DE026259-01A1, R01 DE027980, and R03 DE027147 to A.R.N.; R01 DE02105201A1 to S.N.; and 1K99DE027086 to S.Z.).
The authors deny any conflicts of interest related to this study.
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