Original ResearchKrüppel-like Factor 5 Regulates Stemness, Lineage Specification, and Regeneration of Intestinal Epithelial Stem Cells
Under a Creative Commons license
open access
Graphical abstract
Keywords
Intestinal Stem Cell
Multipotent Differentiation
Tissue Regeneration
Epigenetic Regulation
Abbreviations used in this paper
ASCL2
achaete-scute family bHLH transcription factor 2
ChIP-seq
chromatin immunoprecipitation assay with sequencing
EdU
5-ethynyl-2′-deoxyuridine
EGFP
enhanced green fluorescent protein
GSEA
gene set enrichment analysis
H&E
hematoxylin and eosin
IGV
Integrative Genomics Viewer
ISC
intestinal stem cell
IRR
irradiation
KLF5
Krüppel-like factor 5
LGR5
leucine rich repeat containing G protein-coupled receptor 5
RFP
red fluorescent protein
RNA-seq
RNA sequencing
RT-qPCR
reverse transcriptase quantitative polymerase chain reaction
TA
transit amplifying
TF
transcription factor
TSS
transcription start site
TUNEL
terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick-end labeling
Cited by (0)
Conflicts of interest The authors disclose no conflicts.
Funding This study was supported by National Institutes of Health grants R01DK052230 (to Vincent W. Yang), R01CA084197 (to Vincent W. Yang), R01DK081113 (to Ramesh A. Shivdasani), R01DK082889 (to Ramesh A. Shivdasani), and F32DK115080 (to Madhurima Saxena).
© 2020 The Authors. Published by Elsevier Inc. on behalf of the AGA Institute.