Dissemination of successful international clone ST15 and clonal complex 17 among Bulgarian CTX-M-15 producing K. pneumoniae isolates
Section snippets
Background
Klebsiella pneumoniae is an important bacterial pathogen, causing different hospital and community acquired infections (Wang et al., 2013). The increasing frequency of isolation of highly resistant K. pneumoniae strains is due to the wide distribution of specific epidemic clones, as well as to dissemination of epidemic plasmids (Carattoli, 2013; Woodford et al., 2011). For Bulgaria, European Centre for Disease Prevention and Control (ECDC) reported alarming levels of invasive K. pneumoniae
Material and methods
Between January 2014 and October 2014, 86 consecutive nonduplicated clinically significant Klebsiella isolates (82 K. pneumoniae and 4 Klebsiella oxytoca isolates), resistant to cefotaxime or/and ceftazidime, were obtained from 86 patients hospitalized in Intensive Care Units (ICUs) (n = 27), surgical wards (n = 16), urology wards (n = 19), pediatric wards (n = 5) and internal medicine wards (n = 19) of six Bulgarian hospitals in four towns: Sofia – Medical Institute, Ministry of Interior (310 beds), 20 K.
Results and discussion
All 86 tested isolates exhibited a nonsusceptible phenotype characterized by resistance or intermediate susceptibility (R + I) to aminopenicillins-inhibitor combinations (in 83%), cephalosporins (ceftazidime, 93%; cefotaxime; 100%; cefepime, 100%; cefoxitin 13%), aminoglycosides (tobramycin, 87%; gentamicin, 77%), and fluoroquinolones (ciprofloxacin, 87%; levofloxacin, 63%). Resistance rates to tetracycline and trimethoprim/sulfamethoxazole were 37% and 70%, respectively. The isolates were highly
Financial Support
This study was supported by Contract No 13/2016, Project No. 437/2016 of the Council of Medical Science at the Medical University of Sofia, Bulgaria.
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2023, Microbes and InfectionGenomic analysis of a Kpi (pilus system)-positive and CTX-M-15-producing Klebsiella pneumoniae belonging to the high-risk clone ST15 isolated from an impacted river in Brazil
2022, GenomicsCitation Excerpt :Circular genome map and subsystem obtained from RAST server were accessed (Supplementary Fig. S1). The TIES-4900 strain was assigned to the ST15, which has been recognized as a high-risk international clone causing human infections in hospital settings, European, Asian, African, Oceania, North American and South American countries [3,15–17]. More recently, ESBL-producing K pneumoniae ST15 has been reported in companion animals in Italy [18], France [19], Portugal [20], Germany [21], and Brazil [4], supporting clonal adaptation and persistence of this lineage at the human-animal-environment interface, constituting a One Health issue.
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