Transcriptome profilling analysis characterized the gene expression patterns responded to combined drought and heat stresses in soybean
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Plant materials and stress treatment
The drought-resistant soybean cultivar ‘Heinong44′ was grown in pots with a dimension of 13 × 10 × 10 cm (top diameter × bottom diameter × height), each filled with 1 kg sand. Seeds of ‘Heinong44′ were surface-sterilized in 1% sodium hypochlorite for 10 min, rinsed in distilled water three times, and then sown and grown in a growth chamber at 25°/20 °C (day/night), 14 h/10 h (light/dark), and 60%-80% humidity. Before the V1 stage of development, the seedling was irrigated with 100 mL distilled
Physiological analyses of soybean under drought and heat stress
We studied the physiological response of soybean under heat, drought and combined stress from 0 to 32 h at the V3 stage of development. The SOD activity initially showed a decreasing trend and then increased over time under the heat (H) and combined heat and drought (DH) treatments, and the SOD activity at 8 h was the lowest under both treatment conditions. However, the SOD activity under the drought (D) treatment initially increased and then decreased as the stress time increased, and the
Discussion
Extreme temperature and drought are two major abiotic stresses that often result in significant losses of the yields of crops such as soybean (Tran and Mochida, 2010). Transcriptomic analysis of plant response to stresses is an effective method of discovering key resistance genes, processes and pathways for stress response. The effect of individual drought stress on soybean has been investigated previously by RNA-seq (Chen et al., 2016; Le et al., 2012), while to our knowledge, no report has
Conclusions
Our results showed that many genes are differentially expressed in response to drought and heat, but only a small number of these genes were identified as overlapping DEGs. These overlapping DEGs may play key roles in modulating physiological function in response to stress. Furthermore, comparison of individual and combined heat and drought stress suggests combined stress is not a simple additive mechanism but may involve a complicated gene regulatory network in response to the combination of
Acknowledgments
This work was supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (grant numbers 2014BAD11B01) and the Collaborative Innovation Program of Heilongjiang Soybean Industrial Technology R&D System.
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