Cell
Volume 140, Issue 5, 5 March 2010, Pages 744-752
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An Atlas of Combinatorial Transcriptional Regulation in Mouse and Man

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Summary

Combinatorial interactions among transcription factors are critical to directing tissue-specific gene expression. To build a global atlas of these combinations, we have screened for physical interactions among the majority of human and mouse DNA-binding transcription factors (TFs). The complete networks contain 762 human and 877 mouse interactions. Analysis of the networks reveals that highly connected TFs are broadly expressed across tissues, and that roughly half of the measured interactions are conserved between mouse and human. The data highlight the importance of TF combinations for determining cell fate, and they lead to the identification of a SMAD3/FLI1 complex expressed during development of immunity. The availability of large TF combinatorial networks in both human and mouse will provide many opportunities to study gene regulation, tissue differentiation, and mammalian evolution.

Highlights

► An atlas of human and mouse transcription factor interactions ► Quantification of transcription factor expression across human and mouse tissues ► A network of 15 transcription factors predicts tissue type specification ► SMAD3/FLI1 forms a repressor complex that controls monocyte differentiation

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These authors contributed equally to this work

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Present address: Department of Internal Medicine & Biomedical Engineering, 285 Newton Road, University of Iowa, Iowa City, IA 52242, USA