Biochemical and Biophysical Research Communications
Effects of bone marrow stromal cell injection in an experimental glaucoma model
Section snippets
Materials and methods
Glaucoma model. The use of animals in this study was in accordance with the Guidelines for Animal Experiments of Kyoto University. All animal experiments in this study were conducted with the approval of the Animal Research Committee, Graduate School of Medicine, Kyoto University. Adult female Wistar rats (9 weeks of age) were obtained from Shimizu Laboratory Supplies (Kyoto, Japan). Rats were maintained on a 14 h light/10 h dark cycle and had access to food and water ad libitum.
Chronic IOP
Elevated IOP
The mean and the peak IOP of glaucomatous eyes were significantly elevated compared to those of the sham-operated control eyes (mean IOP; 26.3 ± 2.2 and 19.5 ± 1.7 mmHg, respectively, peak IOP; 28.2 ± 2.0 and 20.9 ± 1.1 mmHg, respectively) (P < 0.01, Student’s t-test). There was no significant difference in the mean and the peak IOP between BMSCs and PBS injected eyes (P > 0.05; Table 1).
Integration of transplanted BMSCs into the glaucomatous retina
Confocal microscopy revealed the existence of GFP-positive BMSCs, in the glaucomatous retina (glaucoma 2 weeks model)one
Discussion
Glaucoma is one of the major causes of blindness in the world, which is characterized by morphological change of the optic disc and the specific loss of RGCs [2]. The goal of glaucoma treatment is to protect the RGCs; however, there is still no direct way to prevent the death of RGCs. In this study, we attempted the transplantation of BMSCs into the glaucoma model rat eye with the aim of compensating for the loss of the RGCs.
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