The Vesicular Monoamine Transporter VMAT2 and Vesicular Acetylcholine Transporter VAChT Are Sorted to Separate Vesicle Populations in PC12 Cells
References (4)
- et al.
Selective storage of ACh, but not catecholamines, in neuroendocrine synaptic-like microvesicles of early endosomal origin
Neuron
(1993) - et al.
Visualization of the vesicular acetylcholine transporter in cholinergic nerve terminals and its targeting to a specific population of small synaptic vesicles
Proc. Natl. Acad. Sci. U.S.A.
(1996)
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Protein sorting in the synaptic vesicle life cycle
2006, Progress in NeurobiologyThe Vesicular Acetylcholine Transporter Interacts with Clathrin-associated Adaptor Complexes AP-1 and AP-2
2004, Journal of Biological ChemistryCitation Excerpt :The dileucine/phosphorylation motif of VAChT may also have a dual function; one of containing an internalization signal, but not via an AP-2 complex, and the other a signal for AP-1-dependent trafficking. An alternative pathway for VAChT trafficking, suggested by Tao-Cheng and Eiden (54) and by Varoque and Erickson (35), utilizes the regulated secretory sorting pathway, as do VMATs, instead of the constitutive secretory pathway, Fig. 10B. Electron microscopy studies showed endogenous VAChT, as well as transfected VAChT, appearing mainly in SLMVs, but also in LDCVs in PC12 cells (1, 35, 54).
Labeling and dynamic imaging of synaptic vesicle-like microvesicles in PC12 cells using TIRFM
2004, Brain ResearchCitation Excerpt :The VAChT is a vesicle-specific, integral membrane protein that transports ACh into synaptic vesicles [10]. VAChT protein appears predominantly in SSVs/SLMVs [6,11,16,18,20] and has been considered an ideal, specific marker of this type of vesicles in various preparations including PC12 cells [6]. The GFP-tagged version of VAChT has been employed to label SLMVs in PC12 cells [12].
The cytoplasmic tail of the vesicular acetylcholine transporter contains a synaptic vesicle targeting signal
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