Trends in Genetics
Technical tipsWhole-mount immunohistochemistry on Xenopus embryos using far-red fluorescent dyes
References (3)
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Methods Cell Biol.
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Cited by (16)
Profiling the in vitro drug-resistance mechanism of influenza A viruses towards the AM2-S31N proton channel blockers
2018, Antiviral ResearchCitation Excerpt :Fluorescence intensity was quantified using Photoshop by measuring the grey scale value. Uninjected oocytes were subjected to the same conditions as described and served as a control for the auto fluorescence from the yolk (Beumer et al., 1995). For each oocyte, the whole-cell current measured by TEVC was plotted against the protein expression level detected by immunofluorescence, and the slope of the obtained linear regression curve represents the relative specific activity of the channel.
Functional studies reveal the similarities and differences between AM2 and BM2 proton channels from influenza viruses
2018, Biochimica et Biophysica Acta - BiomembranesCitation Excerpt :Fluorescence intensity was quantified using Photoshop by measuring the grey scale value. Uninjected oocytes were subjected to the same conditions as described and served as a control for the auto fluorescence from the yolk [50]. CuSO4, CuCl, MnCl2, ZnSO4, MgSO4, and CoSO4 were all purchased from commercial sources.
The Interplay of Functional Tuning, Drug Resistance, and Thermodynamic Stability in the Evolution of the M2 Proton Channel from the Influenza A Virus
2008, StructureCitation Excerpt :Uninjected oocytes were subjected to the above conditions as a control for the autofluorescence from the yolk (Beumer et al., 1995).
Identification of the pore-lining residues of the BM2 ion channel protein of influenza B virus
2008, Journal of Biological ChemistryCitation Excerpt :Intensity quantifications were made using Image Master version 5.0 software (Photon Technologies). Uninjected oocytes were subjected to the same conditions just described as a control for the autofluorescence from the yolk (31). Measurement of the Effects of MTSEA and NEM on the BM2 Cysteine Substitution Mutant Ion Channels—The effect of Cys-specific reagents on BM2 ion channel activities was determined as follows.