Comparative genomic hybridization: Uses and limitations

doi:10.1016/S0037-1963(00)90015-5

Seminars in Hematology

Volume 37, Issue 4, October 2000, Pages 348-357

https://doi.org/10.1016/S0037-1963(00)90015-5 Get rights and content

Abstract

Comparative genomic hybridization (CGH) has contributed significantly to the current knowledge of genomic alterations in hematologic malignancies. Characteristic patterns of genomic imbalances not only have confirmed recent classification schemes in non-Hodgkin's lymphoma, but they provide a basis for the successful identification of genes with previously unrecognized pathogenic roles in the development of different lymphomas. Based on its technical limitations, there is little reason to apply CGH to chromosomes of metaphase cells in routine diagnostic settings. However, the new approach of CGH to DNA microarrays, a procedure termed matrix-CGH, overcomes most of the limitations and opens new approaches for diagnostics and identification of genetically defined leukemia and lymphoma subgroups. Current efforts to develop leukemia specific matrix-CGH DNA chips, which are designed to meet the clinical needs, are presented and discussed.

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Comparative genomic hybridization: Uses and limitations

Abstract

Blood

Blood

Blood

Blood

Blood

Cancer Genet Cytogenet

Trends Genet

Cancer Genet Cytogenet

Blood

Blood

Blood

Cancer Genet Cytogenet

Am J Pathol

Methods Enzymol

Blood

Blood

Cancer Genet Cytogenet

Blood

Am J Pathol

Am J Pathol

Blood

Cancer Genet Cytogenet

Genomics

Cancer Genet Cytogenet

Among numerous DNA copy number changes losses at 13 are highly recurrent in plasmacytoma

Genes Chromosomes Cancer

Low number of DNA copy number changes in small lymphocytic lymphoma

Haematologica

Molecular cytogenetic abnormalities in multiple myeloma and plasma cell leukemia measured using comparative genomic hybridization

Genes Chromosomes Cancer

Comparativegenomic hybridization detects genomic abnormalities in 80% of follicular lymphomas

Br J Haematol

Risk of false positive results in comparative genomic hybridization. Genes Chromosomes

Cancer

Comparative genomic hybridization in the investigation of myeloid leukemias

Genes Chromosomes Cancer

t(11;14)-positive mantle cell lymphomas exhibit complex karyotypes and share similarities with B-cell chronic lymphocytic leukemia

Genes Chromosomes Cancer

Minimal size of deletions detected by comparative genomic hybridization

Genes Chromosomes Cancer

Consistent copy number gain in chromosome 12 in primary diffuse large cell lymphomas of the stomach

Am J Pathol

Characteristic pattern of chromosomal gains and losses in marginal zone Bcell lymphoma detected by comparative genomic hybridization

Leukemia

Cytogenetic and molecular cytogenetic analysis of B cell chronic lymphocytic leukemia: Specific chromosome aberrations identify prognostic subgroups of patients and point to loci of candidate genes

Leukemia

Hardware and software requirements for quantitative analysis of comparative genomic hybridization

Cytometry

Quantitative analysis of comparative genomic hybridization

Cytometry