Environmental endotoxin measurement: The Kinetic Limulus Assay with Resistant-parallel-line Estimation
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Cited by (76)
Lipopolysaccharide Structure and the Phenomenon of Low Endotoxin Recovery
2022, European Journal of Pharmaceutics and BiopharmaceuticsCitation Excerpt :Therefore, for proper working of the LAL assay in hold-time studies, it is required that LPS spikes are recovered from undiluted samples and periodically tested by LAL assays over time.[169] If dilution correction approaches do not reverse the attenuation of the positive control spikes observed over time, then the cause of inadequate LPS detection can be assigned to the concentration-independent and time-dependant effects of LER, otherwise known as masking, which is attributed to the interaction of LPS with solution components impeding LPS detection.[158] Hence, test-interference and LER can be easily experimentally distinguished, Fig. 5.
NIAID, NIEHS, NHLBI, and MCAN Workshop Report: The indoor environment and childhood asthma—implications for home environmental intervention in asthma prevention and management
2017, Journal of Allergy and Clinical ImmunologyCitation Excerpt :For the characterization of indoor microbial communities in dust and air, before the availability of culture-independent technology-enabling metagenomics, environmental microbial taxa were measured by means of either culture, qPCR of select taxa, or quantification of the presence or activity of bioactive indoor pathogen-associated molecular patterns. Gram-negative bacterial endotoxin bioactivity has been quantified by using both kinetic Limulus amebocyte lysate and recombinant Factor C assays.30-32 Endotoxin and the gram-positive pathogen-associated molecular pattern biomarker peptidoglycan (N-acetyl muramic acid) have been also measured by using gas chromatography/mass spectrometry.
Masking of endotoxin in surfactant samples: Effects on Limulus-based detection systems
2016, BiologicalsCitation Excerpt :Notably, approaches which eliminate test interference problems are not effective in overcoming such effects [4]. In the 1990's Greaves and co-workers [11] already differentiated between dilution dependent interference and dilution-independent interference in environmental samples. The latter phenomenon is called masking.
Detecting endotoxin with a flow cytometry-based magnetic aptasensor
2014, Analytical BiochemistryRole of indoor endotoxins in asthma symptoms
2011, Archives des Maladies Professionnelles et de l'EnvironnementRole of indoor endotoxins in asthma symptoms
2010, Revue Francaise d'Allergologie
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Current address: Division of Environmental and Occupational Health, School of Public Health, University of Minnesota, Minneapolis, MN 55455.