Elsevier

Brain Research

Volume 861, Issue 1, 7 April 2000, Pages 165-167
Brain Research

Short communication
α7 Nicotinic receptor-mediated protection against ethanol-induced oxidative stress and cytotoxicity in PC12 cells

https://doi.org/10.1016/S0006-8993(99)02457-9Get rights and content

Abstract

Pheochromocytoma (PC12) cell cultures exhibited a concentration-dependent loss of cells and increase in intracellular oxidative stress when exposed to ethanol for 24 h. Selective activation of α7 nicotinic receptors with the agonist DMXB (3 μM) attenuated both of these actions of ethanol in a manner that was in turn blocked with the nicotinic antagonist methyllyconitine (1 μM). These results suggest that the cytoprotection conferred by α7 nicotinic receptor agonists may be mediated at least in part by reducing the formation or accumulation of reactive oxygen species.

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Acknowledgements

We thank Neal Benton of the University of Florida ICBR for his expert technical support with the flow cytometry, and Dr. John Shryock for helpful discussions of the manuscript. This work was supported in part by T32 AA07561, NIH PO1 10485 and NIAAA RO1 AA 11597.

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