ReviewSlippage and uncoupling in P-type cation pumps; implications for energy transduction mechanisms and regulation of metabolism
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Keywords
P-type ATPase
Slippage
Uncoupling
Variable stoichiometry
Channel
Occluded cation
Partial denaturation
Heat production
Abbreviations
A23187, calcimycin
Ca2+-ATPase, Ca2+- and Mg2+-activated adenosine 5′-triphosphatase (EC 3.6.1.38)
Na+/K+-ATPase, Na+- and K+-activated adenosine 5′-triphosphatase (EC 3.6.1.37)
SR, sarcoplasmic reticulum
E-P, phosphorylated forms of P-type ATPase
E1-P, forms with high affinity cytosolic-orientated cation binding sites
E2-P, forms with low affinity extracytosolic-orientated cation binding sites
TG, thapsigargin
TNP-ATP, 2′(3′)-O-(2,4,6-trinitrophenyl) adenosine-5′-triphosphate
[Ca2+]lim, limiting concentration of medium or cytosolic free calcium ions
EGTA, ethylene bis(oxyethylene nitrilo) tetraacetic acid
DMSO, dimethylsulfoxide
CPA, cyclopiazonic acid
HCPL, high cholesterol proteoliposomes
LCPL, low cholesterol proteoliposomes
NEM, n-methylmaleimide
MGS, molten globular state
SDM, site-directed mutagenesis
SMR, standard metabolic rate
NST, non-shivering thermogenesis
SERCA, sarco- and endoplasmic reticulum Ca2+-ATPase
GABA, γ-aminobutyric acid
K0.5, concentration at half maximal effect
DOPC, dioleoylphosphatidylcholine
AcP, acetylphosphate
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