Effect of motility and chemotaxis on the invasion of Salmonella typhimurium into HeLa cells

doi:10.1016/0882-4010(90)90039-S

Microbial Pathogenesis

Volume 9, Issue 1, July 1990, Pages 47-53

https://doi.org/10.1016/0882-4010(90)90039-S Get rights and content

Abstract

Salmonella typhimurium strain LT2 is able to invade HeLa cells in vitro. The effect of the motility and chemotaxis of the bacteria on cell invasion were examined by two methods: (1) conventional invasion assays where the HeLa cell monolayers were placed horizontally at the bottom of plastic wells and (2) vertical assays where the HeLa cell monolayer attached to one face of plastic bottles was placed vertically. In both assays, the invasion rate of the wild-type strain was higher than that in isogenic non-motile mutants. There was no significant difference between the invasion rate of non-flagellated mutants and that of a flagellated but non-motile mutant. These observations indicated that the motility per se increases the rate of the bacterial invasion by increasing the chance of encounter between Salmonella and the HeLa cells. Smooth-swimming non-chemotactic mutants exhibited 10 times higher invasion rates than the wild-type strain in conventional assays but their invasion rates in vertical assays were approximately equal to that of the wild-type strain. This result indicated that in the conventional assays, the migration of the wild-type bacteria towards the HeLa cells was hampered by their chemotactic responses. Tumbly non-chemotactic mutants exhibited invasion rates intermediate between the wild-type and non-motile strains presumably because of their intermediate net speeds of migration.

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Invasive infections due to foodborne pathogens, including Salmonella enterica serovar Typhimurium, are prevalent and life-threatening. This study aimed to evaluate the effects of ginsenoside Rg3 (Rg3) on the adhesion, invasion, and intracellular survival of S. Typhimurium.
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Rg3 could be considered as a supplement agent to prevent S. Typhimurium infection.
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A possible simple explanation is that each bacterium that reaches the epithelial cell has an equal chance of invading. Previous studies have concluded that flagella and chemotaxis genes are important for the intestinal phase of S. Typhimurium infection, mostly based on studies of the interaction with cultured epithelial cells and mice (Khoramian-Falsafi et al., 1990; Jones et al., 1992; Schmidt et al., 2001). Similar observations have been made for S. Enteritidis flagella mutants in human and avian cell lines (Van Asten et al., 2000; La Ragione et al., 2003) and in the rat (Robertson et al., 2003).
Salmonella enterica serotype Typhimurium (S. Typhimurium) can invade in the intestine of the avian host, and knowledge on the mechanisms that govern this is potentially important for prevention of disease. This study investigated the invasion of S. Typhimurium in the avian host and to which extent it depended on motility and chemotaxis.
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^☆: This work was supported by the Fonds National Suisse de la Recherche Scientifique to JCP (Grant 31-28007.89).

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Microbial Pathogenesis

Abstract

References (15)

Effect of mucin on Campylobacter jejuni association and invasion on Hep-2 cells

Microbial Pathogenesis

Role of chemotaxis in the association of motile bacteria with intestinal mucosa: in vitro studies

Infect Immun

Role of chemotaxis in the association of motile bacteria with intestinal mucosa: in vivo studies

Infect Immun

Motility as virulence factor for Vibrio cholerae

Infect Immun

Flagella, motility and invasive virulence of Pseudomonas aeruginosa

J Gen Microbiol

Flagella of Salmonella typhimurium are a virulence factor in infected C57BL/6J mice

Infect Immun

Flagella help Salmonella typhimurium survive with murine macrophages

Infect Immun

Cited by (67)

Ginsenoside Rg3 reduces the adhesion, invasion, and intracellular survival of Salmonella enterica serovar Typhimurium

Inhibitory potential of Buffalo (Bubalus bubalis) colostrum immunoglobulin G on Klebsiella pneumoniae

TLR1-induced chemokine production is critical for mucosal immunity against Yersinia enterocolitica

Intestinal invasion of Salmonella enterica serovar Typhimurium in the avian host is dose dependent and does not depend on motility and chemotaxis

A flagellated motile Salmonella Gallinarum mutant (SG Fla<sup>+</sup>) elicits a pro-inflammatory response from avian epithelial cells and macrophages and is less virulent to chickens

Effects of prey capture on the swimming and feeding performance of choanoflagellates

ArticleEffect of motility and chemotaxis on the invasion of Salmonella typhimurium into HeLa cells☆

Abstract

Microbial Pathogenesis

Role of chemotaxis in the association of motile bacteria with intestinal mucosa: in vitro studies

Infect Immun

Role of chemotaxis in the association of motile bacteria with intestinal mucosa: in vivo studies

Infect Immun

Motility as virulence factor for Vibrio cholerae

Infect Immun

Flagella, motility and invasive virulence of Pseudomonas aeruginosa

J Gen Microbiol

Flagella of Salmonella typhimurium are a virulence factor in infected C57BL/6J mice

Infect Immun

Flagella help Salmonella typhimurium survive with murine macrophages

Infect Immun

Article
Effect of motility and chemotaxis on the invasion of Salmonella typhimurium into HeLa cells☆