Original contributionEstimation of water content and water mobility in the nucleus and cytoplasm of Xenopus laevis oocytes by NMR microscopy
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Cited by (31)
Single cell spectroscopy: Noninvasive measures of small-scale structure and function
2013, MethodsCitation Excerpt :Although this review is primarily focused on spectroscopic approaches it is worth brief mention of additional spatially resolved biophysical information that can be obtained through NMR imaging (MRI). The possibilities include intracellular structural information [49], maps of water content and mobility [50], water diffusion properties [51], membrane permeability [52], and lipid-specific spin-density maps [51]. Again, due to past and current hardware limitations, single-cell studies have been performed only with the Xenopus oocytes, due to their relatively large diameter.
Osmotic and aging effects in caviar oocytes throughout water and lipid changes assessed by <sup>1</sup>H NMR T<inf>1</inf> and T<inf>2</inf> relaxation and MRI
2007, Magnetic Resonance ImagingCitation Excerpt :A biexponential decay was observed for the T2 relaxation decay of both s and us samples. In agreement with previously reported data [5,14], the T1 measurements of intracellular water yielded to values approximately 50 times higher with respect to the T2 values. Indeed, as is well known, under the extreme narrowing conditions, the theory of dipolar relaxation requires nearly an equality of T1 and T2, as what was reported for pure water.
Subcellular in vivo<sup>1</sup>H MR spectroscopy of Xenopus laevis oocytes
2006, Biophysical JournalCitation Excerpt :To examine the reason for this factor, we compared T2, the spin-spin relaxation times, of the methylene peak at 1.1 ppm in both compartments. Values of 14 ± 1 ms and 12 ± 0.2 ms were obtained for the animal and vegetal cytoplasm, respectively—well in agreement with previously gathered data (12,29). Our in vivo 1H spectra did not reveal metabolites other than triglycerides.
Picoliter <sup>1</sup> H NMR spectroscopy
2002, Journal of Magnetic Resonance