Sequence of the Schizosaccharomyces pombe gtp1 gene and identification of a novel family of putative GTP-binding proteins

doi:10.1016/0378-1119(93)90327-Y

Gene

Volume 125, Issue 2, 30 March 1993, Pages 191-193

https://doi.org/10.1016/0378-1119(93)90327-Y Get rights and content

Abstract

A new gene, gtp1, has been identified by sequence analysis in Schizosaccharomyces pombe. The open reading frame was identified downstream from the stf1 locus. The deduced GTP1 protein has strong sequence similarity to a family of putative GTP-binding proteins from Halobacterium cutirubrum, Bacillus subtilis, Drosophila melanogaster and mouse. The conserved P-loop phosphate-binding motif places gtp1 in a family separate from previously described groups of such proteins.

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There are more references available in the full text version of this article.

Cited by (20)

DRG represents a family of two closely related GTP-binding proteins
2000, Biochimica et Biophysica Acta - Gene Structure and Expression
In a previous publication we identified a novel human GTP-binding protein that was related to DRG, a developmentally regulated GTP-binding protein from the central nervous system of mouse. Here we demonstrate that both the human and the mouse genome possess two closely related drg genes, termed drg1 and drg2. The two genes share 62% sequence identity at the nucleotide and 58% identity at the protein level. The corresponding proteins appear to constitute a separate family within the superfamily of the GTP-binding proteins. The DRG1 and the DRG2 mRNA are widely expressed in human and mouse tissues and show a very similar distribution pattern. The human drg1 gene is located on chromosome 22q12, the human drg2 gene on chromosome 17p12. Distantly related species including Caenorhabditis elegans, Schizosaccharomyces pombe and Saccharomyces cerevisiae also possess two drg genes. In contrast, the genomes of archaebacteria (Halobium, Methanococcus, Thermoplasma) harbor only one drg gene, while eubacteria do not seem to contain any. The high conservation of the polypeptide sequences between distantly related organisms indicates an important role for DRG1 and DRG2 in a fundamental pathway.
Molecular cloning of a novel GTP-binding protein induced in fish cells by rhabdovirus infection
1998, FEBS Letters
We have cloned and sequenced a cDNA encoding GTP-binding protein from a fish cell, CHSE-214. The clone was 1493 bp long and contained an open reading frame encoding 364 amino acids. It has the five sequence motifs G1–G5 that are conserved in all GTP-binding proteins. Its amino acid sequences are strikingly different from those of the well-characterized G-proteins. However, sequences closely related to this protein are found in various kinds of species including human, Arabidopsis, Drosophila and archaebacteria, suggesting a novel subfamily within the superfamily of the GTP-binding proteins. Northern analysis indicates that this gene is constitutively expressed at a low level in normal cells but is induced by fish rhabdovirus infection at about 24 h post infection and disappears thereafter. Based on these observations, we propose that this protein represents an evolutionarily conserved novel subfamily of GTP-binding proteins which may play an important role in fish rhabdovirus infection.
Open reading frames flanking the peptidyl-tRNA hydrolase-encoding gene of Escherichia coli
1994, Gene
The nucleotide (nt) sequences flanking the peptidyl-tRNA hydrolase-encoding gene (pth) of Escherichia coli were determined and analyzed. A coding open reading frame (ORF-3), identified just downstream from pth, had a deduced amino acid (aa) sequence homologous to a family of GTP-binding proteins. We found discrepancies between the ORF-3 sequence from a plasmid clone used in previous studies and another one derived from Kohara's phage collection. Two putative genes, ORF-4 and ORF-2, were also found upstream from pth. ORF-4 could code for a 393-aa peptide homologous to membrane-bound proteins. The nt sequence between ORF-2 and pth revealed the existence of a CAPbinding site correctly positioned to regulate the expression of ORF-2.
Developmentally regulated GTPases: structure, function and roles in disease
2021, Cellular and Molecular Life Sciences
Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule dynamics
2017, Scientific Reports
The universally conserved prokaryotic GTPases
2011, Microbiology and Molecular Biology Reviews

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^∗: Present address: J.D. Hudson, Cold Spring Harbor Laboratories, Cold Spring Harbor, NY 11729, USA.

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Abstract

References (12)

: The P-loop — a common motif in ATP- and GTP-binding proteins

TIBS

ras genes

Annu. Rev. Biochem.

stf1: non-wee mutations epistatic to cdc25 in the fission yeast Schizosaccharomyces pombe

Genetics

Structure and function of signal-transducing GTP-binding proteins

Annu. Rev. Biochem.

Primary structure and functional aspects of the gene coding for the second-largest subunit of RNA polymerase III of Drosophila

Mol. Gen. Genet.

G protein multiplicity in eukaryotic signal transduction systems

Biochemistry

Cited by (20)

DRG represents a family of two closely related GTP-binding proteins

Molecular cloning of a novel GTP-binding protein induced in fish cells by rhabdovirus infection

Open reading frames flanking the peptidyl-tRNA hydrolase-encoding gene of Escherichia coli

Developmentally regulated GTPases: structure, function and roles in disease

Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule dynamics

The universally conserved prokaryotic GTPases

Short communicationSequence of the Schizosaccharomyces pombe gtp1 gene and identification of a novel family of putative GTP-binding proteins

Abstract

TIBS

ras genes

Annu. Rev. Biochem.

stf1: non-wee mutations epistatic to cdc25 in the fission yeast Schizosaccharomyces pombe

Genetics

Structure and function of signal-transducing GTP-binding proteins

Annu. Rev. Biochem.

Primary structure and functional aspects of the gene coding for the second-largest subunit of RNA polymerase III of Drosophila

Mol. Gen. Genet.

G protein multiplicity in eukaryotic signal transduction systems

Biochemistry

Short communication
Sequence of the Schizosaccharomyces pombe gtp1 gene and identification of a novel family of putative GTP-binding proteins