An experimental model to evaluate the in vivo response of rat seminal vesicle to electrical stimulation

doi:10.1016/0304-3940(96)12354-5

Neuroscience Letters

Volume 204, Issue 3, 9 February 1996, Pages 215-217

https://doi.org/10.1016/0304-3940(96)12354-5 Get rights and content

Abstract

This investigation demonstrated a good model to record the pressure change of rat seminal vesicle (SV) in response to electrical stimulation (ES) of lesser splanchnic nerve (LSN) in vivo. LSN was easily identified and isolated grossly. Antegrade catheterization via the SV tail to the main lumen was performed similar to the technique of i.v catheterization, and the pressure recording was quite satisfactory. The vesicle response to ES was frequency-dependent and reproducible. Under the conditions of 10 V and 1 ms, the maximal vesicle pressure was 81.7±2.8 mmHg with 80 Hz of ES. The inhibitory response of SV to ES by clomipramine was concentration-dependent. Clomipramine (3 mg/kg) could inhibit 53% of control responses (n = 7). This experimental model is relevant to neurophysiologic and neuropharmacologic studies of SV.

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Cited by (12)

Parasympathetic influence plays an independent and significant role in inducing the contraction of the seminal vesicle of the rat
2010, Urology
Citation Excerpt :
Mature male Wistar rats, aged 12-14 weeks, were obtained from the animal center of National Taiwan University Medical College. After the rats were anesthetized with pentobarbital (40 mg/kg) via intraperitoneal injections, they were used for in vivo animal experiments as previously described.5 In brief, the trachea of the rat was intubated to keep the airway patent.
The role of the parasympathetic pathway in seminal vesicle (SV) contraction has not been well described. The purpose of this study was to study parasympathetic effects, the dominant muscarinic receptors subtype(s), and nitric oxide (NO) effects for SV contraction.
In vivo, SV pressure of mature male Wistar rats were recorded after electric stimulation (ES) of each pelvic nerve (PN; parasympathetic pathway) alone; bilateral PNs simultaneously, the L6 and S1 branches of the left PN; the left PN after ablation of sympathetic influence; the lesser splanchnic nerve (LSN) after ablation of parasympathetic influence; and the LSN after pretreatment of 4 muscarinic receptor antagonists or a NO donor—3-Morpholinosydnonimine (SIN-1).
ES to the left PN caused frequency-dependent SV contraction, with similar results after ES to the right PN and bilateral PNS. ES to the L6 branch of the left PN caused significantly greater SV response than to the S1 branch. Ablation of sympathetic influence did not affect SV response to parasympathetic stimulation and vice versa. The inhibitory effects of 4-DAMP (M3 antagonist) and atropine (nonselective muscarinic antagonist) on SV response to ES were similar and significantly greater than those of pirenzepine (M1 antagonist) and methoctramine (M2 antagonist). Pretreatment of SIN-1 partially suppressed the SV response of ES to left PN.
ES via the parasympathetic pathway independently induces contraction of rat SV; NO partially suppresses the SV pressure response to parasympathetic ES.
Premature ejaculation
2010, Revista Internacional de Andrologia
La eyaculación prematura es considerada la disfunción sexual más frecuente en el varón. Aunque su prevalencia real no es bien conocida, ya que la falta hasta ahora de una definición aceptada unánimemente permite cuestionar los criterios de inclusión y valoración de muchos de los estudios publicados, los análisis con mayor significación estadística la cifran en un 30%. Según los datos del primer Estudio Demográfico Español sobre Eyaculación Precoz (DEEP), dados a conocer en diciembre de 2009, el 43% de los españoles sufre de eyaculación prematura en algún momento de su vida. Ante la necesidad de crear un estándar universal y en respuesta a la diversidad de definiciones existentes, la Sociedad Internacional de Medicina Sexual (ISSM) ha propuesto una nueva definición basada en criterios objetivos. Existen suficientes evidencias para sugerir el origen biogénico de la eyaculación prematura, determinado genéticamente y en relación con la sensibilidad heredada de los receptores centrales de la serotonina. Hasta ahora no se disponía de un fármaco específicamente diseñado para el tratamiento de la eyaculación prematura, por lo que los fármacos utilizados se prescribían off label. La aprobación, por las agencias española (AEMPS) y europea (EMEA) de Medicamentos, de dapoxetina, un inhibidor selectivo de la recaptación de serotonina de acción corta, supone un importante avance en el tratamiento de la eyaculación prematura. Este artículo revisa los principales aspectos en relación con la definición, la epidemiología, la etiología, la clasificación y las opciones terapéuticas de la eyaculación prematura.
Premature ejaculation is considered the most common male sexual dysfunction. Although its real prevalence is not well known, due to the current absence of a universal accepted definition which allows questioning the inclusion criteria and assessment of many published studies, the analyses with higher statistical significance report 30%. According to the data of the first Spanish Demographic Study on Premature Ejaculation (DEEP), revealed in December 2009, 43% of Spaniards suffers from premature ejaculation in any moment of his life. Because of the need to create an universal standard in response to the diversity of existent definitions, the International Society of Sexual Medicine (ISSM) has proposed a new definition based on objective criteria. There are evidences enought that suggest the biogenic origin of the premature ejaculation, genetically determined and in relation to the inherited sensitivity of the central serotonine receptors. So far, there was not any specific available a drug designed for the treatment of premature ejaculation. Therefore, the most common practice was to prescribe “off label” drugs. The European (EMEA) and Spanish (AEMPS) medicaments agencies approved the use of dapoxetine, a short-acting selective serotonin reuptake inhibitor. This is an important advance in the treatment of premature ejaculation. This article reviews the main aspects regarding the definition, epidemiology, etiology, classification and treatment options of premature ejaculation.
Effects of various nitric oxide-donating drugs on adrenergic tension of human seminal vesicles in vitro
2003, Urology
To evaluate the effects of the nitric oxide (NO)-donating compounds sodium nitroprusside (SNP), S-nitroso-glutathione (GSNO), S-nitroso-N-acetylcysteine (SNAC), S-nitroso-N-acetylcysteine-ethylester (SNACET), and linsidomine (SIN-1) on the adrenergic tension of isolated human seminal vesicle strip preparations. The significance of the NO-cyclic guanosine monophosphate (cGMP) pathway in the regulation of smooth muscle tone in the human genitourinary tract has been well established. However, information on the significance of NO-mediated signal transduction in the functional control of the mammalian seminal vesicles is still sparse.
Seminal vesicle strip preparations were applied to an organ bath system under standard conditions. Tension was induced by the addition of 10 μM norepinephrine. After stable tension plateaus had been reached, the drugs were added in a cumulative manner (0.01 to 100 μM) and the isometric responses of the tissue registered. The effects of the compounds on the phasic contractility of the tissue preparations were also evaluated. The adenylyl cyclase-stimulating agent forskolin was used as a reference compound known to interfere with the cyclic adenosine monophosphate pathway.
Adrenergic tension was dose dependently attenuated by the drugs. The rank order of potency, from greater to lesser, was GSNO, SNAC, SNP, SIN-1, forskolin, and SNACET. The rank order (from greater to lesser) with regard to the inhibitory effects of the compounds on the frequency of phasic contractions of the tissue induced by the addition of norepinephrine was GSNO, SNAC, SNP, and SIN-1; the effects of SIN-1, forskolin, and SNACET on the frequency of contractions were nearly equipotent.
Our results strongly support the hypothesis that the contractility of the human seminal vesicle is under the control of the NO-cGMP pathway. This finding may give a rationale for the use of S-nitrosothiols, such as GSNO and SNAC, in the pharmacotherapy of hyperexcitatory disturbances of ejaculation (premature ejaculation).
Effects of various nitric oxide donating agents on the contractility and cyclic nucleotide turnover of human seminal vesicles in vitro
2002, Urology
Objectives. To evaluate the effects of the nitric oxide (NO)-donating drugs sodium nitroprusside, S-nitroso-glutathione (GSNO), S-nitroso-N-acetylcysteineetylester (SNACET), and linsidomine (SIN-1), as well as the adenylyl cyclase-stimulating agent forskolin, on electrically induced contractions and on tissue levels of cyclic guanosine monophosphate (cGMP) and cyclic adenosine monophosphate (cAMP) of isolated human seminal vesicle strip preparations. The significance of the l-arginine-NO-cGMP pathway in the regulation of smooth muscle tone in the human genitourinary tract has been well established; however, information on the relevance of NO-mediated signal transduction in the functional control of mammalian seminal vesicles is still sparse.
Methods. Seminal vesicle strip preparations were applied to an organ bath system under standard conditions. Phasic contractions were induced by electrical field stimulation (frequency 80 Hz, amplitude 10 V, single pulse 1 ms, total pulse duration 1 second, pause 90 seconds). After stable contraction amplitudes had been reached, the drugs were added in a cumulative manner (0.001 to 10 μM), and the isometric responses were registered. After drug exposure, freezing, tissue homogenization, and extraction of cyclic nucleotides, cAMP and cGMP were measured by means of enzyme-linked immunosorbent assays.
Results. Electrical field stimulation-induced amplitudes were attenuated by the drugs in a dose-dependent manner. The rank order of potency was GSNO > sodium nitroprusside > forskolin > SNACET ≥ SIN-1. The relaxing effect of GSNO was antagonized in the presence of 10 μM of guanylyl cyclase inhibitor methylene blue. The inhibitory effects of GSNO, sodium nitroprusside, and forskolin on the contractile activity were paralleled by an increase in tissue cGMP (2 to 100-fold) and cAMP (7 to 9-fold).
Conclusions. Our results strongly support the hypothesis that the contractility of human seminal vesicles is in part regulated by the NO-cGMP-cascade. This may give a rationale for the use of S-nitrosothiols, such as GSNO, in the pharmacotherapy of hyperexcitatory disturbances of ejaculation.
An ex vivo evaluation of regulatory role of biogenic amines in rat seminal vesicle after pharmacological manipulation
1998, Life Sciences
We studied the neural regulation of seminal vesicles (SV) by determining the changes of intraluminal pressure of rat SV in response to an electric stimulation of the lesser splanchnic nerve (LSN). After pharmacological manipulation with neurotoxin, the contents of monoamines and their metabolites in SV were estimated. In rats receiving electric stimulation of the LSN, an increase of intraluminal pressure was obtained with a reduction of the serotonergic turnover rate in SV. An intraperitoneal injection of DSP-4 (100 $mg kg$ ), the noradrenergic neurotoxin, into rats decreased the level of norepinephrine (NE) in SV significantly but did not influence this functional response. Also, the intraluminal pressure was lowered by an intrathecal injection of 6-OHDA (20 $μg rat$ ) to denervate spinal monoaminergic nerves in rats although the contents of monoamines in SV were not changed. This indicates that noradrenergic neurotransmission appears unimportant in this regulation. The lowering of intraluminal pressure in rats by 6-OHDA is mainly related to an attenuation of dopaminergic neuroregulation from the decrease of turnover rate of DA. Otherwise, an intrathecal injection of 5,7-DHT (60 $μg rat$ ) to abolish spinal serotonergic nerves did not influence the level of monoamines in SV but increased the intraluminal pressure indicating an involvement of inhibitory regulation from spinal serotonergic pathway. These results suggest that contraction by electric stimulation of the LSN in Wistar rat SV is mainly regulated by the dopaminergic nervous pathway and an inhibitory regulation of the serotonergic nervous pathway from spinal cord while the noradrenergic nervous system seems unimportant for this regulation.
Neuroanatomy and neurophysiology related to sexual dysfunction in male neurogenic patients with lesions to the spinal cord or peripheral nerves
2010, Spinal Cord

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^☆: This study was supported by the National Science Council Grant NSC 85-2331-B-002-032.

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An experimental model to evaluate the in vivo response of rat seminal vesicle to electrical stimulation☆

Abstract

Brain Res.

The role of clomipramine in the treatment of premature ejaculation

The vesicle sphincter and ejaculation in the ram

Br. J. Urol.

Motor innervation of the smooth muscle of the rat seminal vesicle

J. Pharmacol. Exp. Ther.