Adaptation of the rapid automated tetrazolium dye based (MTT) assay for chemosensitivity testing in childhood leukemia

doi:10.1016/0304-3835(88)90294-7

Cancer Letters

Volume 41, Issue 3, 30 August 1988, Pages 323-332

https://doi.org/10.1016/0304-3835(88)90294-7 Get rights and content

Abstract

The reduction of the tetrazolium salt MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) to a blue-black formazan product by living but not by dead cells can be used to measure chemosensitivity of tumor cells. The main advantages of the MTT assay are its simplicity, rapidity, and the fact that the results are read automatically with a microplate spectrophotometer. Several reports on the use of the MTT assay in chemosensitivity testing have been published, but all these studies dealt with established cell lines and not with specimens obtained directly from patients. Here we present a study in which the MTT assay has been adapted to assess the effect of antineoplastic drugs on lymphoblasts of children with leukemia.

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Research letterAdaptation of the rapid automated tetrazolium dye based (MTT) assay for chemosensitivity testing in childhood leukemia

Abstract

Leuk. Res.

J. Immunol. Methods

Eur. J. Cancer Clin. Oncol.

J. Immunol. Methods.

J. Immunol. Methods

J. Immunol. Methods

J. Immunol. Methods

Validation of an automated microculture tetrazolium assay (MTA) to assess growth and drug sensitivity of human tumor cell lines

Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay

Cancer Res.

Research letter
Adaptation of the rapid automated tetrazolium dye based (MTT) assay for chemosensitivity testing in childhood leukemia