Elsevier

Antiviral Research

Volume 11, Issues 5–6, June–July 1989, Pages 263-273
Antiviral Research

Isolation, purification, and partial characterization of prunellin, an anti-HIV component from aqueous extracts of Prunella vulgaris

https://doi.org/10.1016/0166-3542(89)90036-3Get rights and content

Abstract

Prunellin, an anti-HIV active compound, was isolated from aqueous extracts of the Chinese medicinal herb, Prunella vulgaris, and purified to chromatographic homogeneity. Infrared and NMR spectroscopy identified prunellin as a polysaccharide. Elemental analyses, precipitation with calcium(II), barium(II), or 9-aminoacridine suggest a sulfated polysaccharide. Paper chromatography of the exhaustively hydrolyzed material indicates the presence of glucose, galactose, xylose, gluconic acid, galactonic acid and galactosamine as the constituent monosaccharides. The molecular size of prunellin, as determined by gel permeation chromatography and the Squire method on Sephadex G-75, is about 10 kDa.

References (21)

There are more references available in the full text version of this article.

Cited by (103)

  • Potential of selected Lamiaceae plants in anti(retro)viral therapy

    2018, Pharmacological Research
    Citation Excerpt :

    This is not the only polysaccharide with detected anti-HIV properties. Investigating inflorescence of self-heal, researchers were able to isolate sulfated polysaccharide named prunellin, with excellent activity against HIV, without detected cytotoxicity [54,98]. Furthermore, various active compounds were isolated from different representatives of genus Salvia and tested for antiviral properties.

  • Structural characterization and antiviral effect of a novel polysaccharide PSP-2B from Prunellae Spica

    2016, Carbohydrate Polymers
    Citation Excerpt :

    PS has also been utilized clinically to treat high blood pressure, breast hyperplasia, hyperthyrea, and herpetic keratitis (Cui & Miao, 2014; Gao & Dong, 2003; Yang, Guo, Wu, Ye, & Xia, 2007). Phytochemical studies have illustrated that PS is rich in triterpenoids (Kojima & Ogura, 1986; Ryu et al., 2000), polysaccharides (Dore et al., 2013; Tabba, Chang, & Smith, 1989; Xu, Lee, Lee, White, & Blay, 1999), phenolic acids (Liu, 2010), flavonoids (Cheung & Zhang, 2008; Ye, 2010) and steroids (Kojima, Sato, Hatano, & Ogura, 1990). Pharmacological investigations have also indicated that the crude aqueous extract of PS has immunostimulatory, antioxidant, anti-inflammatory, antitumor and antiviral activities (Choi et al., 2010; Han et al., 2009; Tabba et al., 1989; Xu et al., 1999).

  • Phytochemistry and pharmacological activities of the genus Prunella

    2016, Food Chemistry
    Citation Excerpt :

    The anti-HIV effect of P. vulgaris water extract occurs mainly during the early interference and late viral binding events (Oh, Price, Brindley, et al., 2011), by inhibiting recombinant HIV-1 reverse transcriptase activity, with ID50 of 26.0 μg/ml (Collins, Ng, Fong, Wan, & Yeung, 1997). A sulfur-containing anionic polysaccharide-prunellin with a molecular weight of 10 kDa isolated from the water extract of P. vulgaris showed the ability to block HIV-1 from entering cells (Tabba et al., 1989). Some studies reported that the major anti-HIV-1 effective substance was tannins of P. vulgaris and that they worked by inhibiting HIV-1 integrase activity (Au, Lam, Ng, Fong, & Wan, 2001), as well as inhibiting the formation of the six-helix bundle of HIV-1 gp41 protein (Liu et al., 2002) and the recombinant HIV-1 protease activity (Lam et al., 2000).

View all citing articles on Scopus
View full text