Establishment and characterisation of murine cells constitutively expressing the fusion, nucleoprotein and matrix proteins of measles virus

https://doi.org/10.1016/0166-0934(93)90055-VGet rights and content

Abstract

To advance our understanding of the immunobiology of measles virus (MV) infections, we have investigated the possibility of establishing cell lines constitutively expressing the individual MV antigens. In contrast to previously published studies, we show that it is possible to establish cell lines expressing high levels of fusion (F), nucleoprotein (NP) and matrix (M) MV proteins. Once cloned, the cell lines were stable with high levels of expression for more than six months. The size and cell distribution of the NP and F proteins were similar to those observed in MV- or vaccinia-MV recombinant-infected cells. In contrast, the distribution of the M protein, although being similar to that of MV-infected cells, differed from that of Vaccinia-M recombinant virus-infected cells. Preliminary results suggest that these cell lines will be useful tools for studying the contribution of individual MV antigens to the cell-mediated immune response to this virus.

References (33)

  • B. Bankamp et al.

    Measles virus nucleocapsid protein protects rats from encephalitis

    J. Virol.

    (1991)
  • P. Bertolino et al.

    Correlation between invariant chain expression level and capability to present antigen to MHC class II-restricted T cells

    Int. Imm.

    (1991)
  • D. Blondel et al.

    Role of matrix protein in cytopathogenesis of vesicular stomatitis virus

    J. Virol.

    (1990)
  • R. Buckland et al.

    Cloning of the matrix gene of measles virus (Halley strain)

    Nucleic Acids Res.

    (1990)
  • R. Buckland et al.

    A Ieucine zipper structure present in the measles virus fusion protein is not required for its tetramerisation but is essential for fusion

    J. gen. Vir.

    (1992)
  • C. Chen et al.

    High-efficiency transformation of mammalian cells by plasmid DNA

    Mol. Cell. Biol.

    (1987)
  • Cited by (24)

    View all citing articles on Scopus
    View full text