Research paperA method for fixation of previously fresh-frozen human adult and fetal brains that preserves histological quality and immunoreactivity
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2016, Journal of Psychiatric ResearchCitation Excerpt :As for other factors such as treatment and alcohol comorbidity, we acknowledge the possibility of this having an effect on the results, but the nature of the sample collection restrains the sample size so we chose to include all subjects available that passed the pH/agonal factor criteria to avoid losing analysis power. Following the method described by Jones et al (1992) the brains were removed during autopsy, cooled down and cut in the coronal plane into 0.75 cm slabs, which were then frozen and stored at −80 °C. To ensure the selection of samples adequate for gene expression analysis, brain tissue pH and agonal factors were evaluated following the procedure described by Tomita et al. (2004).
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2014, Schizophrenia ResearchCitation Excerpt :All subjects that were clinically characterized with the psychological autopsy method died suddenly without prolonged agonal state. The human brain dissection and freezing protocol are described in detail elsewhere (Jones et al., 1992). RNA was prepared from 80 to 100 mg of frozen tissue samples, with Omni Prep Multi-Sample Homogenizer (Omni International, Kennesaw, GA).
Glutamate transporters: A key piece in the glutamate puzzle of major depressive disorder
2013, Journal of Psychiatric ResearchCitation Excerpt :Control subjects had no history of psychiatric or neurological disorders nor any in their first degree relatives. The brains were removed during autopsy and cut in the coronal plane into 0.75 cm slabs (Jones et al., 1992). The slabs were then frozen and stored at −80 °C until needed.
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